Skip to main content

Research Repository

Advanced Search

All Outputs (19)

Development of resident and migratory three-spined stickleback, Gasterosteus aculeatus (2024)
Journal Article
Barnes, M., Chakrabarti, L., & MacColl, A. D. C. (2024). Development of resident and migratory three-spined stickleback, Gasterosteus aculeatus. PLoS ONE, 19(7), Article e0295485. https://doi.org/10.1371/journal.pone.0295485

The three-spined stickleback (Gasterosteus aculeatus) is a teleost fish and a model organism in evolutionary ecology, useful for both laboratory and natural experiments. It is especially valued for the substantial intraspecific variation in morpholog... Read More about Development of resident and migratory three-spined stickleback, Gasterosteus aculeatus.

Second-generation antipsychotics and metabolic syndrome: a role for mitochondria (2023)
Journal Article
Mortimer, K. R. H., Katshu, M. Z. U. H., & Chakrabarti, L. (2023). Second-generation antipsychotics and metabolic syndrome: a role for mitochondria. Frontiers in Psychiatry, 14, Article 1257460. https://doi.org/10.3389/fpsyt.2023.1257460

Psychosis is a known risk factor for developing metabolic syndrome (MetS). The risk is even greater in patients who are taking second-generation antipsychotics (SGAs). SGAs exacerbate metabolic abnormalities and lead to a 3-fold increased risk of sev... Read More about Second-generation antipsychotics and metabolic syndrome: a role for mitochondria.

A method to assess the mitochondrial respiratory capacity of complexes I and II from frozen tissue using the Oroboros O2k-FluoRespirometer (2023)
Journal Article
Ebanks, B., Kwiecinska, P., Moisoi, N., & Chakrabarti, L. (2023). A method to assess the mitochondrial respiratory capacity of complexes I and II from frozen tissue using the Oroboros O2k-FluoRespirometer. PLoS ONE, 18(7), Article e0276147. https://doi.org/10.1371/journal.pone.0276147

High-resolution respirometry methods allow for the assessment of oxygen consumption by the electron transfer systems within cells, tissue samples, and isolated mitochondrial preparations. As mitochondrial integrity is compromised by the process of cr... Read More about A method to assess the mitochondrial respiratory capacity of complexes I and II from frozen tissue using the Oroboros O2k-FluoRespirometer.

A Common Anaesthetic, MS-222, Alters Measurements Made Using High-Resolution Respirometry in the Three-Spined Stickleback (Gasterosteus aculeatus) (2023)
Journal Article
Barnes, M., Ebanks, B., MacColl, A., & Chakrabarti, L. (2023). A Common Anaesthetic, MS-222, Alters Measurements Made Using High-Resolution Respirometry in the Three-Spined Stickleback (Gasterosteus aculeatus). Fishes, 8(1), Article 42. https://doi.org/10.3390/fishes8010042

Submersion in the anaesthetic MS-222 is a well-established and effective method used during the euthanasia of fish, but the consequences of treatment with this anaesthetic for mitochondrial respiration are yet to be established. This is important to... Read More about A Common Anaesthetic, MS-222, Alters Measurements Made Using High-Resolution Respirometry in the Three-Spined Stickleback (Gasterosteus aculeatus).

Mitochondrial ATP Synthase is a Target of Oxidative Stress in Neurodegenerative Diseases (2022)
Journal Article
Ebanks, B., & Chakrabarti, L. (2022). Mitochondrial ATP Synthase is a Target of Oxidative Stress in Neurodegenerative Diseases. Frontiers in Molecular Biosciences, 9, Article 854321. https://doi.org/10.3389/fmolb.2022.854321

The mitochondrial ATP synthase is responsible for the production of cellular ATP, and it does so by harnessing the membrane potential of the mitochondria that is produced by the sequential oxidation of select cellular metabolites. Since the structura... Read More about Mitochondrial ATP Synthase is a Target of Oxidative Stress in Neurodegenerative Diseases.

Sox-positive cell population in the adult cerebellum increases upon tissue degeneration (2021)
Journal Article
Salih, S., Nizamudeen, Z. A., De Melo, N., Chakrabarti, L., & Sottile, V. (2022). Sox-positive cell population in the adult cerebellum increases upon tissue degeneration. Experimental Neurology, 348, Article 113950. https://doi.org/10.1016/j.expneurol.2021.113950

Adult neurogenesis is well-described in the subventricular and subgranular zones of the mammalian brain. Recent observations that resident glia express stem cell markers in some areas of the brain not traditionally associated with neurogenesis hint t... Read More about Sox-positive cell population in the adult cerebellum increases upon tissue degeneration.

Exercising D. melanogaster Modulates the Mitochondrial Proteome and Physiology. The Effect on Lifespan Depends upon Age and Sex (2021)
Journal Article
Ebanks, B., Wang, Y., Katyal, G., Sargent, C., Ingram, T. L., Bowman, A., …Chakrabarti, L. (2021). Exercising D. melanogaster Modulates the Mitochondrial Proteome and Physiology. The Effect on Lifespan Depends upon Age and Sex. International Journal of Molecular Sciences, 22(21), Article 11606. https://doi.org/10.3390/ijms222111606

Ageing is a major risk factor for many of the most prevalent diseases, including neurode-generative diseases, cancer, and heart disease. As the global population continues to age, behavioural interventions that can promote healthy ageing will improve... Read More about Exercising D. melanogaster Modulates the Mitochondrial Proteome and Physiology. The Effect on Lifespan Depends upon Age and Sex.

Sequence and structure comparison of ATP synthase F0 subunits 6 and 8 in notothenioid fish (2021)
Journal Article
Katyal, G., Ebanks, B., Lucassen, M., Papetti, C., & Chakrabarti, L. (2021). Sequence and structure comparison of ATP synthase F0 subunits 6 and 8 in notothenioid fish. PLoS ONE, 16(10), 1-18. https://doi.org/10.1371/journal.pone.0245822

Mitochondrial changes such as tight coupling of the mitochondria have facilitated sustained oxygen and respiratory activity in haemoglobin-less icefish of the Channichthyidae family. We aimed to characterise features in the sequence and structure of... Read More about Sequence and structure comparison of ATP synthase F0 subunits 6 and 8 in notothenioid fish.

The dysregulated Pink1- drosophila mitochondrial proteome is partially corrected with exercise (2021)
Journal Article
Ebanks, B., Ingram, T. L., Katyal, G., Ingram, J. R., Moisoi, N., & Chakrabarti, L. (2021). The dysregulated Pink1- drosophila mitochondrial proteome is partially corrected with exercise. Aging, 13(11), 14709-14728. https://doi.org/10.18632/aging.203128

One of the genes which has been linked to the onset of juvenile/early onset Parkinson’s disease (PD) is PINK1. There is evidence that supports the therapeutic potential of exercise in the alleviation of PD symptoms. It is possible that exercise may e... Read More about The dysregulated Pink1- drosophila mitochondrial proteome is partially corrected with exercise.

ATP synthase and Alzheimer's disease: putting a spin on the mitochondrial hypothesis (2020)
Journal Article
Ebanks, B., Ingram, T. L., & Chakrabarti, L. (2020). ATP synthase and Alzheimer's disease: putting a spin on the mitochondrial hypothesis. Aging, 12(6), 16647—16662. https://doi.org/10.18632/aging.103867

It is estimated that over 44 million people across the globe have dementia, and half of these cases are believed to be Alzheimer's disease (AD). As the proportion of the global population which is over the age 60 increases so will the number of indiv... Read More about ATP synthase and Alzheimer's disease: putting a spin on the mitochondrial hypothesis.

New Approaches to Tay-Sachs Disease Therapy (2018)
Journal Article
Solovyeva, V. V., Shaimardanova, A. A., Chulpanova, D. S., Kitaeva, K. V., Chakrabarti, L., & Rizvanov, A. A. (2018). New Approaches to Tay-Sachs Disease Therapy. Frontiers in Physiology, 9(NOV), Article 1663. https://doi.org/10.3389/fphys.2018.01663

Tay-Sachs disease belongs to the group of autosomal-recessive lysosomal storage metabolic disorders. This disease is caused by β-hexosaminidase A (HexA) enzyme deficiency due to various mutations in α-subunit gene of this enzyme, resulting in GM2 gan... Read More about New Approaches to Tay-Sachs Disease Therapy.

Elevated 5hmC levels characterize DNA of the cerebellum in Parkinson’s disease (2017)
Journal Article
Stöger, R., Scaife, P., Shephard, F., & Chakrabarti, L. (in press). Elevated 5hmC levels characterize DNA of the cerebellum in Parkinson’s disease. npj Parkinson's Disease, 3(6), https://doi.org/10.1038/s41531-017-0007-3

5-methylcytosine and the oxidation product 5-hydroxymethylcytosine are two prominent epigenetic variants of the cytosine base in nuclear DNA of mammalian brains. We measured levels of 5-methylcytosine and 5-hydroxymethylcytosine by enzyme-linked immu... Read More about Elevated 5hmC levels characterize DNA of the cerebellum in Parkinson’s disease.

Proteomic profiling of mitochondria: what does it tell us about the ageing brain? (2016)
Journal Article
Ingram, T., & Chakrabarti, L. (2016). Proteomic profiling of mitochondria: what does it tell us about the ageing brain?. Aging, 8(12), https://doi.org/10.18632/aging.101131

Mitochondrial dysfunction is evident in numerous neurodegenerative and age-related disorders. It has also been linked to cellular ageing, however our current understanding of the mitochondrial changes that occur are unclear. Functional studies have m... Read More about Proteomic profiling of mitochondria: what does it tell us about the ageing brain?.

Mitochondrial complex 1 activity measured by spectrophotometry is reduced across all brain regions in ageing and more specifically in neurodegeneration (2016)
Journal Article
Cai, H., Pollard, A. K., Craig, E. L., & Chakrabarti, L. (in press). Mitochondrial complex 1 activity measured by spectrophotometry is reduced across all brain regions in ageing and more specifically in neurodegeneration. PLoS ONE, 11(6), Article e0157405. https://doi.org/10.1371/journal.pone.0157405

Mitochondrial function, in particular complex 1 of the electron transport chain (ETC), has been shown to decrease during normal ageing and in neurodegenerative disease. However, there is some debate concerning which area of the brain has the greatest... Read More about Mitochondrial complex 1 activity measured by spectrophotometry is reduced across all brain regions in ageing and more specifically in neurodegeneration.

Analysis of mitochondrial haemoglobin in Parkinson's disease brain (2016)
Journal Article
Shephard, F., Greville-Heygate, O., Liddell, S., Emes, R. D., & Chakrabarti, L. (2016). Analysis of mitochondrial haemoglobin in Parkinson's disease brain. Mitochondrion, 29, 45-52. https://doi.org/10.1016/j.mito.2016.05.001

Mitochondrial dysfunction is an early feature of neurodegeneration. We have shown there are mitochondrial haemoglobin changes with age and neurodegeneration. We hypothesised that altered physiological processes are associated with recruitment and loc... Read More about Analysis of mitochondrial haemoglobin in Parkinson's disease brain.