Development of resident and migratory three-spined stickleback, Gasterosteus aculeatus

Barnes, Megan; Chakrabarti, Lisa; MacColl, Andrew D C

Abstract

The three-spined stickleback (Gasterosteus aculeatus) is a teleost fish and a model organism in evolutionary ecology, useful for both laboratory and natural experiments. It is especially valued for the substantial intraspecific variation in morphology, behaviour and genetics. Classic work of Swarup (1958) has described the development in the laboratory of embryos from a single freshwater population, but this was carried out at higher temperature than many stickleback would encounter in the wild and variation between populations was not addressed. Here we describe the development of embryos from two sympatric, saltwater ecotypes of stickleback from North Uist, Scotland raised at 14°C, the approximate temperature of North Uist lochs in the breeding season. The two ecotypes were (a) a large, migratory form in which the adults are completely plated with bony armour and (b) a smaller, low-plated form that is resident year-round in saltwater lagoons. By monitoring embryos every 24-hours post fertilisation, important characteristics of development were observed and photographed to provide a reference for North Uist ecotypes at this temperature. Hatching success was greater than 85% and did not differ between resident and migratory stickleback, but migratory eggs hatched significantly earlier than the resident ecotype. Our work provides a framework that can now be used to compare stickleback populations that may also grow in distinct environmental conditions, to help understand the breadth of normal developmental features and to characterise abnormal development.

Citation

Barnes, M., Chakrabarti, L., & MacColl, A. D. C. (2024). Development of resident and migratory three-spined stickleback, Gasterosteus aculeatus. PLoS ONE, 19(7), Article e0295485. https://doi.org/10.1371/journal.pone.0295485

• Journal Article Type: Article
• Acceptance Date: Jun 5, 2024
• Online Publication Date: Jul 18, 2024
• Publication Date: Jul 18, 2024
• Deposit Date: Jul 30, 2024
• Publicly Available Date: Jul 30, 2024
• Journal: PLoS ONE
• Electronic ISSN: 1932-6203
• Publisher: Public Library of Science
• Peer Reviewed: Peer Reviewed
• Volume: 19
• Issue: 7
• Article Number: e0295485
• DOI: https://doi.org/10.1371/journal.pone.0295485
• Keywords: Sticklebacks; Bird eggs; Hatching success; Clutches; Embryos; Lagoons; Fertilization; Yolk sac
• Public URL: https://nottingham-repository.worktribe.com/output/37320146
• Publisher URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0295485