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Sequence and structure comparison of ATP synthase F0 subunits 6 and 8 in notothenioid fish

Katyal, Gunjan; Ebanks, Brad; Lucassen, Magnus; Papetti, Chiara; Chakrabarti, Lisa

Authors

Gunjan Katyal

Brad Ebanks

Magnus Lucassen

Chiara Papetti



Abstract

Mitochondrial changes such as tight coupling of the mitochondria have facilitated sustained oxygen and respiratory activity in haemoglobin-less icefish of the Channichthyidae family. We aimed to characterise features in the sequence and structure of the proteins directly involved in proton transport, which have potential physiological implications. ATP synthase subunit a (ATP6) and subunit 8 (ATP8) are proteins that function as part of the F0 component (proton pump) of the F0F1complex. Both proteins are encoded by the mitochondrial genome and involved in oxidative phosphorylation. To explore mitochondrial sequence variation for ATP6 and ATP8 we analysed sequences from C. gunnari and C. rastrospinosus and compared them with their closely related red-blooded species and eight other vertebrate species. Our comparison of the amino acid sequence of these proteins reveals important differences that could underlie aspects of the unique physiology of the icefish. In this study we find that changes in the sequence of subunit a of the icefish C. gunnari at position 35 where there is a hydrophobic alanine which is not seen in the other notothenioids we analysed. An amino acid change of this type is significant since it may have a structural impact. The biology of the haemoglobin-less icefish is necessarily unique and any insights about these animals will help to generate a better overall understanding of important physiological pathways.

Citation

Katyal, G., Ebanks, B., Lucassen, M., Papetti, C., & Chakrabarti, L. (2021). Sequence and structure comparison of ATP synthase F0 subunits 6 and 8 in notothenioid fish. PLoS ONE, 16(10), 1-18. https://doi.org/10.1371/journal.pone.0245822

Journal Article Type Article
Acceptance Date Sep 9, 2021
Online Publication Date Oct 6, 2021
Publication Date Oct 6, 2021
Deposit Date Oct 13, 2021
Publicly Available Date Oct 13, 2021
Journal PLOS ONE
Electronic ISSN 1932-6203
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 16
Issue 10
Article Number e0245822
Pages 1-18
DOI https://doi.org/10.1371/journal.pone.0245822
Public URL https://nottingham-repository.worktribe.com/output/6457463
Publisher URL https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0245822

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