All Outputs (27)

Mapping Polyclonal Antibody Responses to Infection Using Next-Generation Phage Display (2023)
Book Chapter
Tsoumpeli, M. T., Varghese, A., Owen, J. P., Maddison, B. C., Daly, J. M., & Gough, K. C. (2023). Mapping Polyclonal Antibody Responses to Infection Using Next-Generation Phage Display. In M. Hust, & T. S. Lim (Eds.), Phage Display (467-487). Springer US. https://doi.org/10.1007/978-1-0716-3381-6_25

Peptide phage display has historically been used to epitope map monoclonal antibodies. More recently, by coupling this method with next-generation sequencing (so-called next-generation phage display, NGPD) to mass screen peptide binding events, the m... Read More about Mapping Polyclonal Antibody Responses to Infection Using Next-Generation Phage Display.

Recombinant ovine prion protein can be mutated at position 136 to improve its efficacy as an inhibitor of prion propagation (2023)
Journal Article
Kopycka, K., Maddison, B. C., & Gough, K. C. (2023). Recombinant ovine prion protein can be mutated at position 136 to improve its efficacy as an inhibitor of prion propagation. Scientific Reports, 13(1), Article 3452. https://doi.org/10.1038/s41598-023-30202-0

Prion diseases are progressive neurodegenerative disorders with no effective therapeutics. The central event leading to the pathology in the diseases is the conversion of PrPC into PrPSc and its accumulation in the central nervous system. Previous st... Read More about Recombinant ovine prion protein can be mutated at position 136 to improve its efficacy as an inhibitor of prion propagation.

Therapeutic Phage Display-Derived Single-Domain Antibodies for Pandemic Preparedness (2023)
Journal Article
Daly, J. M., Lim, T. S., & Gough, K. C. (2023). Therapeutic Phage Display-Derived Single-Domain Antibodies for Pandemic Preparedness. Antibodies, 12(1), Article 7. https://doi.org/10.3390/antib12010007

Driven by necessity, the COVID-19 pandemic caused by SARS-CoV-2 has accelerated the development and implementation of new vaccine platforms and other viral therapeutics. Among these is the therapeutic use of antibodies including single-domain antibod... Read More about Therapeutic Phage Display-Derived Single-Domain Antibodies for Pandemic Preparedness.

Next-Generation Phage Display to Identify Peptide Ligands of Deubiquitinases (2022)
Book Chapter
Spiliotopoulos, A., Maurer, S. K., Tsoumpeli, M. T., Bonfante, J. A. F., Owen, J. P., Gough, K. C., & Dreveny, I. (2023). Next-Generation Phage Display to Identify Peptide Ligands of Deubiquitinases. In J. Maupin-Furlow, & M. J. Edelmann (Eds.), Deubiquitinases: Methods and Protocols (189-218). Humana Press. https://doi.org/10.1007/978-1-0716-2803-4_12

Phage display (PD) is a powerful method and has been extensively used to generate monoclonal antibodies and identify epitopes, mimotopes, and protein interactions. More recently, the combination of next-generation sequencing (NGS) with PD (NGPD) has... Read More about Next-Generation Phage Display to Identify Peptide Ligands of Deubiquitinases.

A Simple Whole-Plasmid PCR Method to Construct High-Diversity Synthetic Phage Display Libraries (2022)
Journal Article
Tsoumpeli, M. T., Gray, A., Parsons, A. L., Spiliotopoulos, A., Owen, J. P., Bishop, K., …Gough, K. C. (2022). A Simple Whole-Plasmid PCR Method to Construct High-Diversity Synthetic Phage Display Libraries. Molecular Biotechnology, 64, 791-803. https://doi.org/10.1007/s12033-021-00442-4

Phage display technology utilises peptide and antibody libraries with very high diversities to select ligands with specific binding properties. The production of such libraries can be labour intensive and technically challenging and whilst there are... Read More about A Simple Whole-Plasmid PCR Method to Construct High-Diversity Synthetic Phage Display Libraries.

BSE can propagate in sheep co-infected or pre-infected with scrapie (2021)
Journal Article
Chong, A., Foster, J. D., Goldmann, W., Gonzalez, L., Jeffrey, M., O'Connor, M. J., …Hunter, N. (2021). BSE can propagate in sheep co-infected or pre-infected with scrapie. Scientific Reports, 11, Article 11931. https://doi.org/10.1038/s41598-021-91397-8

To understand the possible role of mixed-prion infections in disease presentation, the current study reports the co-infection of sheep with bovine spongiform encephalopathy (BSE) and scrapie. The bovine BSE agent was inoculated subcutaneously into sh... Read More about BSE can propagate in sheep co-infected or pre-infected with scrapie.

Advances in the Production and Batch Reformatting of Phage Antibody Libraries (2019)
Journal Article
Reader, R. H., Workman, R. G., Maddison, B. C., & Gough, K. C. (2019). Advances in the Production and Batch Reformatting of Phage Antibody Libraries. Molecular Biotechnology, 61, 801-815. https://doi.org/10.1007/s12033-019-00207-0

Phage display antibody libraries have proven an invaluable resource for the isolation of diagnostic and potentially therapeutic antibodies, the latter usually being antibody fragments converted into IgG formats. Recent advances in the production of h... Read More about Advances in the Production and Batch Reformatting of Phage Antibody Libraries.

BSE infectivity survives burial for five years with only limited spread (2019)
Journal Article
Somerville, R. A., Fernie, K., Smith, A., Bishop, K., Maddison, B. C., Gough, K. C., …Gough, K. C. (2019). BSE infectivity survives burial for five years with only limited spread. Archives of Virology, 164(4), 1135–1145. https://doi.org/10.1007/s00705-019-04154-8

© 2019, The Author(s). The carcasses of animals infected with bovine spongiform encephalopathy (BSE), scrapie or chronic wasting disease (CWD) that remain in the environment (exposed or buried) may continue to act as reservoirs of infectivity. We co... Read More about BSE infectivity survives burial for five years with only limited spread.

Needle in a haystack? A comparison of eDNA metabarcoding and targeted qPCR for detection of the great crested newt (Triturus cristatus) (2018)
Journal Article
Harper, L. R., Lawson Handley, L., Hahn, C., Boonham, N., Rees, H. C., Gough, K. C., …Hänfling, B. (2018). Needle in a haystack? A comparison of eDNA metabarcoding and targeted qPCR for detection of the great crested newt (Triturus cristatus). Ecology and Evolution, 8(12), 6330-6341. https://doi.org/10.1002/ece3.4013

Environmental DNA (eDNA) analysis is a rapid, cost-effective, non-invasive biodiversity monitoring tool which utilises DNA left behind in the environment by organisms for species detection. The method is used as a species-specific survey tool for rar... Read More about Needle in a haystack? A comparison of eDNA metabarcoding and targeted qPCR for detection of the great crested newt (Triturus cristatus).

The detection of great crested newts year round via environmental DNA analysis (2017)
Journal Article
Rees, H. C., Baker, C. A., Gardner, D. S., Maddison, B. C., & Gough, K. C. (2017). The detection of great crested newts year round via environmental DNA analysis. BMC Research Notes, 10(1), Article 327. https://doi.org/10.1186/s13104-017-2657-y

Objective Analysis of environmental DNA (eDNA) is a method that has been used for the detection of various species within water bodies. The great crested newt (Triturus cristatus) has a short eDNA survey season (mid-April to June). Here we investi... Read More about The detection of great crested newts year round via environmental DNA analysis.

An in vitro model for assessing effective scrapie decontamination (2017)
Journal Article
Gough, K. C., Baker, C., & Maddison, B. (2017). An in vitro model for assessing effective scrapie decontamination. Veterinary Microbiology, 207, https://doi.org/10.1016/j.vetmic.2017.05.018

Scrapie infectivity enters the environment via a multiplicity of routes from infected animals. Environmentally associated scrapie persists on farms when infected animals have been removed and is particularly resistant to disinfection. Infectivity wit... Read More about An in vitro model for assessing effective scrapie decontamination.

How is Europe positioned for a re-emergence of Schmallenberg virus? (2017)
Journal Article
Tarlinton, R., Gough, K., Maddison, B., Stavrou, A., Daly, J. M., Maddison, B. C., …Tarlinton, R. E. (2017). How is Europe positioned for a re-emergence of Schmallenberg virus?. Veterinary Journal, 230, 45-51. https://doi.org/10.1016/j.tvjl.2017.04.009

The Schmallenberg virus (SBV) caused a large scale epidemic in Europe from 2011–2013 infecting ruminants and causing fetal deformities after infection of pregnant animals. The main impacts of the virus were financial losses due to animal, meat and se... Read More about How is Europe positioned for a re-emergence of Schmallenberg virus?.

In vitro amplification of H-type atypical bovine spongiform encephalopathy by protein misfolding cyclic amplification (2017)
Journal Article
O'Connor, M., Bishop, K., Workman, R. G., Maddison, B. C., & Gough, K. C. (in press). In vitro amplification of H-type atypical bovine spongiform encephalopathy by protein misfolding cyclic amplification. Prion, 11(1), 54-64. https://doi.org/10.1080/19336896.2016.1259051

The in vitro amplification of prions by serial protein misfolding cyclic amplification has been shown to detect PrPSc to levels at least as sensitive as rodent bioassay but in a fraction of the time. Bovine spongiform encephalopathy is a zoonotic pri... Read More about In vitro amplification of H-type atypical bovine spongiform encephalopathy by protein misfolding cyclic amplification.

A sensitive 301V BSE serial PMCA assay (2016)
Journal Article
Gough, K. C., Bishop, K., Somerville, R. A., Hunter, N., & Maddison, B. C. (2016). A sensitive 301V BSE serial PMCA assay. F1000Research, 5(2529), https://doi.org/10.12688/f1000research.9735.1

The prion strain 301V, is a mouse passaged form of bovine spongiform encephalopathy (BSE). It has been used as a model of BSE for more than 20 years, in particular in the investigation of tissue distribution of infectivity, the molecular phenotype an... Read More about A sensitive 301V BSE serial PMCA assay.

Methods for differentiating prion types in food-producing animals (2015)
Journal Article
Gough, K. C., Rees, H. C., Ives, S. E., & Maddison, B. C. (2015). Methods for differentiating prion types in food-producing animals. Biology, 4(4), https://doi.org/10.3390/biology4040785

Prions are an enigma amongst infectious disease agents as they lack a genome yet confer specific pathologies thought to be dictated mainly, if not solely, by the conformation of the disease form of the prion protein (PrPSc). Prion diseases affect hum... Read More about Methods for differentiating prion types in food-producing animals.

Objects in contact with classical scrapie sheep act as a reservoir for scrapie transmission (2015)
Journal Article
Konold, T., Hawkins, S. A., Thurston, L. C., Maddison, B. C., Gough, K. C., Duarte, A., & Simmons, H. A. (2015). Objects in contact with classical scrapie sheep act as a reservoir for scrapie transmission. Frontiers in Veterinary Science, 2, Article 32. https://doi.org/10.3389/fvets.2015.00032

Classical scrapie is an environmentally transmissible prion disease of sheep and goats. Prions can persist and remain potentially infectious in the environment for many years and thus pose a risk of infecting animals after re-stocking. In vitro studi... Read More about Objects in contact with classical scrapie sheep act as a reservoir for scrapie transmission.

Evidence for a novel Kit adhesion domain mediating human mast cell adhesion to structural airway cells (2015)
Journal Article
Gough, K. C., Maddison, B. C., Shikotra, A., Moiseeva, E. P., Yang, W., Jarvis, S., & Bradding, P. (2015). Evidence for a novel Kit adhesion domain mediating human mast cell adhesion to structural airway cells. Respiratory Research, 16, Article 86. https://doi.org/10.1186/s12931-015-0245-z

Background: Human lung mast cells (HLMCs) infiltrate the airway epithelium and airway smooth muscle (ASM) in asthmatic airways. The mechanism of HLMC adhesion to both cell types is only partly defined, and adhesion is not inhibited by function-blocki... Read More about Evidence for a novel Kit adhesion domain mediating human mast cell adhesion to structural airway cells.

Applications and limitations of measuring environmental DNA as indicators of the presence of aquatic animals (2015)
Journal Article
Rees, H. C., Gough, K. C., Middleditch, D. J., Patmore, J. R., & Maddison, B. C. (2015). Applications and limitations of measuring environmental DNA as indicators of the presence of aquatic animals. Journal of Applied Ecology, 52(4), https://doi.org/10.1111/1365-2664.12467

1. In Rees et al. (2014b), we reviewed the current status of environmental DNA (eDN A) tomonitor aquatic populations. Our aim was to focus on discus sion of methodologies used,application of eDNA analysis as a survey tool in ecology, and to include s... Read More about Applications and limitations of measuring environmental DNA as indicators of the presence of aquatic animals.

Does the Presence of Scrapie Affect the Ability of Current Statutory Discriminatory Tests To Detect the Presence of Bovine Spongiform Encephalopathy? (2015)
Journal Article
Maddison, B. C., Gough, K. C., Bishop, K., Simmons, M. M., Chaplin, M. J., Vickery, C. M., …Spiropoulos, J. (2015). Does the Presence of Scrapie Affect the Ability of Current Statutory Discriminatory Tests To Detect the Presence of Bovine Spongiform Encephalopathy?. Journal of Clinical Microbiology, 53(8), 2593-2604. https://doi.org/10.1128/jcm.00508-15

Current European Commission (EC) surveillance regulations require discriminatory testing of all transmissible spongiform encephalopathy (TSE)-positive small ruminant (SR) samples in order to classify them as bovine spongiform encephalopathy (BSE) or... Read More about Does the Presence of Scrapie Affect the Ability of Current Statutory Discriminatory Tests To Detect the Presence of Bovine Spongiform Encephalopathy?.

Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrPSc over time (2015)
Journal Article
Maddison, B. C., Spiropoulos, J., Vickery, C. M., Lockey, R., Owen, J. P., Bishop, K., …Gough, K. C. (2015). Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrPSc over time. Veterinary Research, 46, Article 46. https://doi.org/10.1186/s13567-015-0179-y

Ovine scrapie can be transmitted via environmental reservoirs. A pool of ovine scrapie isolates were incubated on soil for one day or thirteen months and eluted prion was used to challenge tg338 mice transgenic for ovine PrP. After one-day incubation... Read More about Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrPSc over time.