Advances in the Production and Batch Reformatting of Phage Antibody Libraries

Reader, Rose H.; Workman, Robert G.; Maddison, Ben C.; Gough, Kevin C.

Abstract

Phage display antibody libraries have proven an invaluable resource for the isolation of diagnostic and potentially therapeutic antibodies, the latter usually being antibody fragments converted into IgG formats. Recent advances in the production of highly diverse and functional antibody libraries are considered here, including for Fabs, scFvs and nanobodies. These advances include codon optimisation during generation of CDR diversity, improved display levels using novel signal sequences, molecular chaperones and isomerases and the use of highly stable scaffolds with relatively high expression levels. In addition, novel strategies for the batch reformatting of scFv and Fab phagemid libraries, derived from phage panning, into IgG formats are described. These strategies allow the screening of antibodies in the end-use format, facilitating more efficient selection of potential therapeutics.

Citation

Reader, R. H., Workman, R. G., Maddison, B. C., & Gough, K. C. (2019). Advances in the Production and Batch Reformatting of Phage Antibody Libraries. Molecular Biotechnology, 61, 801-815. https://doi.org/10.1007/s12033-019-00207-0

• Journal Article Type: Article
• Acceptance Date: Aug 15, 2019
• Online Publication Date: Aug 29, 2019
• Publication Date: Aug 29, 2019
• Deposit Date: Aug 30, 2019
• Publicly Available Date: Aug 30, 2019
• Journal: Molecular Biotechnology
• Print ISSN: 1073-6085
• Electronic ISSN: 1559-0305
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 61
• Pages: 801-815
• DOI: https://doi.org/10.1007/s12033-019-00207-0
• Keywords: Biotechnology; Applied Microbiology and Biotechnology; Biochemistry; Bioengineering; Molecular Biology
• Public URL: https://nottingham-repository.worktribe.com/output/2520895
• Publisher URL: https://link.springer.com/article/10.1007/s12033-019-00207-0
• Contract Date: Aug 30, 2019