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Advances in the Production and Batch Reformatting of Phage Antibody Libraries

Reader, Rose H.; Workman, Robert G.; Maddison, Ben C.; Gough, Kevin C.

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Authors

Rose H. Reader

Robert G. Workman

Ben C. Maddison

KEVIN GOUGH KEVIN.GOUGH@NOTTINGHAM.AC.UK
Professor of Biochemistry and Pathology



Abstract

Phage display antibody libraries have proven an invaluable resource for the isolation of diagnostic and potentially therapeutic antibodies, the latter usually being antibody fragments converted into IgG formats. Recent advances in the production of highly diverse and functional antibody libraries are considered here, including for Fabs, scFvs and nanobodies. These advances include codon optimisation during generation of CDR diversity, improved display levels using novel signal sequences, molecular chaperones and isomerases and the use of highly stable scaffolds with relatively high expression levels. In addition, novel strategies for the batch reformatting of scFv and Fab phagemid libraries, derived from phage panning, into IgG formats are described. These strategies allow the screening of antibodies in the end-use format, facilitating more efficient selection of potential therapeutics.

Journal Article Type Article
Acceptance Date Aug 15, 2019
Online Publication Date Aug 29, 2019
Publication Date Aug 29, 2019
Deposit Date Aug 30, 2019
Publicly Available Date Aug 30, 2019
Journal Molecular Biotechnology
Print ISSN 1073-6085
Electronic ISSN 1559-0305
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 61
Pages 801-815
DOI https://doi.org/10.1007/s12033-019-00207-0
Keywords Biotechnology; Applied Microbiology and Biotechnology; Biochemistry; Bioengineering; Molecular Biology
Public URL https://nottingham-repository.worktribe.com/output/2520895
Publisher URL https://link.springer.com/article/10.1007/s12033-019-00207-0

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