Research Repository

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The chronic stages of bovine Fasciola hepatica are dominated by CD4 T-Cell exhaustion (2017)
Journal Article
Sachdev, D., Gough, K. C., & Flynn, R. J. (2017). The chronic stages of bovine Fasciola hepatica are dominated by CD4 T-Cell exhaustion. Frontiers in Immunology, 8, doi:10.3389/fimmu.2017.01002. ISSN 1664-3224

Fasciola hepatica infection of ruminants leads to non-resolving chronic infection, as patency develops, there is switching to a TGF-β and IL-10 led response. Here, we explore the responses of CD4 T-cells within the major draining lymph nodes. We foun... Read More

The detection of great crested newts year round via environmental DNA analysis (2017)
Journal Article
Rees, H. C., Baker, C. A., Gardner, D. S., Maddison, B. C., & Gough, K. C. (2017). The detection of great crested newts year round via environmental DNA analysis. BMC Research Notes, 10(1), doi:10.1186/s13104-017-2657-y. ISSN 1756-0500

Objective Analysis of environmental DNA (eDNA) is a method that has been used for the detection of various species within water bodies. The great crested newt (Triturus cristatus) has a short eDNA survey season (mid-April to June). Here we investi... Read More

Ovine recombinant PrP as an inhibitor of ruminant prion propagation in vitro (2017)
Journal Article
Maddison, B. C., Workman, R. G., & Gough, K. C. (in press). Ovine recombinant PrP as an inhibitor of ruminant prion propagation in vitro. Prion, 11(4), doi:10.1080/19336896.2017.1342919. ISSN 1933-6896

Prion diseases are fatal and incurable neurodegenerative diseases of humans and animals. Despite years of research, no therapeutic agents have been developed that can effectively manage or reverse disease progression. Recently it has been identified... Read More

An in vitro model for assessing effective scrapie decontamination (2017)
Journal Article
Gough, K. C., Baker, C., & Maddison, B. (2017). An in vitro model for assessing effective scrapie decontamination. Veterinary Microbiology, 207, doi:10.1016/j.vetmic.2017.05.018. ISSN 0378-1135

Scrapie infectivity enters the environment via a multiplicity of routes from infected animals. Environmentally associated scrapie persists on farms when infected animals have been removed and is particularly resistant to disinfection. Infectivity wit... Read More

How is Europe positioned for a re-emergence of Schmallenberg virus? (2017)
Journal Article
Stavrou, A., Daly, J. M., Maddison, B. C., Gough, K. C., & Tarlinton, R. E. (2017). How is Europe positioned for a re-emergence of Schmallenberg virus?. Veterinary Journal, 230, doi:10.1016/j.tvjl.2017.04.009. ISSN 1532-2971

The Schmallenberg virus (SBV) caused a large scale epidemic in Europe from 2011–2013 infecting ruminants and causing fetal deformities after infection of pregnant animals. The main impacts of the virus were financial losses due to animal, meat and se... Read More

In vitro amplification of H-type atypical bovine spongiform encephalopathy by protein misfolding cyclic amplification (2017)
Journal Article
O'Connor, M., Bishop, K., Workman, R. G., Maddison, B. C., & Gough, K. C. (in press). In vitro amplification of H-type atypical bovine spongiform encephalopathy by protein misfolding cyclic amplification. Prion, 11(1), doi:10.1080/19336896.2016.1259051. ISSN 1933-6896

The in vitro amplification of prions by serial protein misfolding cyclic amplification has been shown to detect PrPSc to levels at least as sensitive as rodent bioassay but in a fraction of the time. Bovine spongiform encephalopathy is a zoonotic pri... Read More

A sensitive 301V BSE serial PMCA assay (2016)
Journal Article
Gough, K. C., Bishop, K., Somerville, R. A., Hunter, N., & Maddison, B. C. (2016). A sensitive 301V BSE serial PMCA assay. F1000Research, 5(2529), doi:10.12688/f1000research.9735.1. ISSN 2046-1402

The prion strain 301V, is a mouse passaged form of bovine spongiform encephalopathy (BSE). It has been used as a model of BSE for more than 20 years, in particular in the investigation of tissue distribution of infectivity, the molecular phenotype an... Read More

Mapping B-cell responses to Salmonella enterica serovars Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals (2016)
Journal Article
Typhimurium and Enteritidis in chickens for the discrimination of infected from vaccinated animals. Scientific Reports, 6, doi:10.1038/srep31186. ISSN 2045-2322

Serological surveillance and vaccination are important strategies for controlling infectious diseases of food production animals. However, the compatibility of these strategies is limited by a lack of assays capable of di erentiating infected from va... Read More

Mapping polyclonal antibody responses to bacterial infection using next generation phage display (2016)
Journal Article
Naqid, I. A., Owen, J. P., Maddison, B. C., Spiliotopoulos, A., Emes, R. D., Warry, A., …Gough, K. C. (2016). Mapping polyclonal antibody responses to bacterial infection using next generation phage display. Scientific Reports, 6, doi:10.1038/srep24232. ISSN 2045-2322

Mapping polyclonal antibody responses to infectious diseases to identify individual epitopes has the potential to underpin the development of novel serological assays and vaccines. Here, phage-peptide library panning coupled with screening using next... Read More

Methods for differentiating prion types in food-producing animals (2015)
Journal Article
Gough, K. C., Rees, H. C., Ives, S. E., & Maddison, B. C. (2015). Methods for differentiating prion types in food-producing animals. Biology, 4(4), doi:10.3390/biology4040785. ISSN 2079-7737

Prions are an enigma amongst infectious disease agents as they lack a genome yet confer specific pathologies thought to be dictated mainly, if not solely, by the conformation of the disease form of the prion protein (PrPSc). Prion diseases affect hum... Read More

Objects in contact with classical scrapie sheep act as a reservoir for scrapie transmission (2015)
Journal Article
Konold, T., Hawkins, S. A., Thurston, L. C., Maddison, B. C., Gough, K. C., Duarte, A., & Simmons, H. A. (2015). Objects in contact with classical scrapie sheep act as a reservoir for scrapie transmission. Frontiers in Veterinary Science, 2, doi:10.3389/fvets.2015.00032. ISSN 2297-1769

Classical scrapie is an environmentally transmissible prion disease of sheep and goats. Prions can persist and remain potentially infectious in the environment for many years and thus pose a risk of infecting animals after re-stocking. In vitro studi... Read More

Evidence for a novel Kit adhesion domain mediating human mast cell adhesion to structural airway cells (2015)
Journal Article
Gough, K. C., Maddison, B. C., Shikotra, A., Moiseeva, E. P., Yang, W., Jarvis, S., & Bradding, P. (2015). Evidence for a novel Kit adhesion domain mediating human mast cell adhesion to structural airway cells. Respiratory Research, 16, doi:10.1186/s12931-015-0245-z. ISSN 1465-9921

Background: Human lung mast cells (HLMCs) infiltrate the airway epithelium and airway smooth muscle (ASM) in asthmatic airways. The mechanism of HLMC adhesion to both cell types is only partly defined, and adhesion is not inhibited by function-blocki... Read More

Applications and limitations of measuring environmental DNA as indicators of the presence of aquatic animals (2015)
Journal Article
Rees, H. C., Gough, K. C., Middleditch, D. J., Patmore, J. R., & Maddison, B. C. (2015). Applications and limitations of measuring environmental DNA as indicators of the presence of aquatic animals. Journal of Applied Ecology, 52(4), doi:10.1111/1365-2664.12467. ISSN 0021-8901

1. In Rees et al. (2014b), we reviewed the current status of environmental DNA (eDN A) tomonitor aquatic populations. Our aim was to focus on discus sion of methodologies used,application of eDNA analysis as a survey tool in ecology, and to include s... Read More

Sensitive recovery of recombinant antibody clones after their in silico identification within NGS datasets (2015)
Journal Article
Spiliotopoulos, A., Owen, J. P., Maddison, B. C., Dreveny, I., Rees, H. C., & Gough, K. C. (2015). Sensitive recovery of recombinant antibody clones after their in silico identification within NGS datasets. Journal of Immunological Methods, 420, doi:10.1016/j.jim.2015.03.005. ISSN 0022-1759

Recently the analytical power of the latest high throughput next generation DNA sequencing platforms has been used to analyse phage that have been selected from the panning of large combinatorial libraries displaying either peptide or antibody ligand... Read More