A novel D2O tracer method to quantify RNA turnover as a biomarker of de novo ribosomal biogenesis, in vitro, in animal models, and in human skeletal muscle

Brook, Matthew S.; Wilkinson, D.J.; Mitchell, W. Kyle; Lund, Jonathan N.; Phillips, Bethan E.; Szewczyk, Nathaniel J.; Kainulainen, H.; Lensu, S.; Koch, L.G.; Britton, S.L.; Greenhaff, Paul L.; Smith, K.; Atherton, Philip J.

doi:10.1152/ajpendo.00157.2017

All Outputs (2)

Human skeletal muscle is refractory to the anabolic effects of leucine during the postprandial muscle-full period in older men (2017)
Journal Article

Leucine modulates muscle protein synthesis (MPS), with potential to facilitate accrual/maintenance of muscle mass. Animal models suggest that leucine boluses shortly after meals may prolong MPS and delay onset of a “muscle-full” state. However, the e... Read More about Human skeletal muscle is refractory to the anabolic effects of leucine during the postprandial muscle-full period in older men.

A novel D2O tracer method to quantify RNA turnover as a biomarker of de novo ribosomal biogenesis, in vitro, in animal models, and in human skeletal muscle (2017)
Journal Article

Current methods to quantify in vivo RNA dynamics are limited. Here, we developed a novel stable isotope (D2O) methodology to quantify RNA synthesis (i.e., ribosomal biogenesis) in cells, animal models, and humans. First, proliferating C2C12 cells wer... Read More about A novel D2O tracer method to quantify RNA turnover as a biomarker of de novo ribosomal biogenesis, in vitro, in animal models, and in human skeletal muscle.