All Outputs (10)

Scalable bioreactor production of an O2‐protected [FeFe]‐hydrogenase enables simple aerobic handling for clean chemical synthesis (2024)
Journal Article

The enzyme CbA5H, a [FeFe]-hydrogenase from Clostridium beijerinckii, has previously been shown to survive exposure to oxygen, making it a promising candidate for biotechnological applications. Thus far [NiFe]-hydrogenases are typically considered fo... Read More about Scalable bioreactor production of an O2‐protected [FeFe]‐hydrogenase enables simple aerobic handling for clean chemical synthesis.

Developing a genetic engineering method for Acetobacterium wieringae to expand one-carbon valorization pathways (2023)
Journal Article

Background: Developing new bioprocesses to produce chemicals and fuels with reduced production costs will greatly facilitate the replacement of fossil-based raw materials. In most fermentation bioprocesses, the feedstock usually represents the highes... Read More about Developing a genetic engineering method for Acetobacterium wieringae to expand one-carbon valorization pathways.

A primer to directed evolution: current methodologies and future directions (2023)
Journal Article

Directed evolution is one of the most powerful tools for protein engineering and functions by harnessing natural evolution, but on a shorter timescale. It enables the rapid selection of variants of biomolecules with properties that make them more sui... Read More about A primer to directed evolution: current methodologies and future directions.

A combinatorial DNA assembly approach to biosynthesis of N-linked glycans in E. coli (2023)
Journal Article

Glycoengineering of recombinant glycans and glycoconjugates is a rapidly evolving field. However, the production and exploitation of glycans has lagged behind that of proteins and nucleic acids. Biosynthetic glycoconjugate production requires the coo... Read More about A combinatorial DNA assembly approach to biosynthesis of N-linked glycans in E. coli.

Complete Genome Sequence of Paraclostridium bifermentans DSM 14991 (2022)
Journal Article

The complete genome sequence of Paraclostridium bifermentans was obtained by assembly of Illumina and Oxford Nanopore (ONT) reads. The sequence will enable study into the organism’s ability to biohydrogenate unsaturated acyl chains in the transformat... Read More about Complete Genome Sequence of Paraclostridium bifermentans DSM 14991.

Versatile selective evolutionary pressure using synthetic defect in universal metabolism (2021)
Journal Article

The non-natural needs of industrial applications often require new or improved enzymes. The structures and properties of enzymes are difficult to predict or design de novo. Instead, semi-rational approaches mimicking evolution entail diversification... Read More about Versatile selective evolutionary pressure using synthetic defect in universal metabolism.

Combinatorial assembly platform enabling engineering of genetically stable metabolic pathways in cyanobacteria (2021)
Journal Article

Cyanobacteria are simple, efficient, genetically-tractable photosynthetic microorganisms which in principle represent ideal biocatalysts for CO2 capture and conversion. However, in practice, genetic instability and low productivity are key, linked pr... Read More about Combinatorial assembly platform enabling engineering of genetically stable metabolic pathways in cyanobacteria.

Combinatorial metabolic engineering platform enabling stable overproduction of lycopene from carbon dioxide by cyanobacteria (2020)
Other

Cyanobacteria are simple, efficient, genetically-tractable photosynthetic microorganisms representing ideal biocatalysts for CO2 capture and conversion, in principle. In practice, genetic instability and low productivity are key, linked problems in e... Read More about Combinatorial metabolic engineering platform enabling stable overproduction of lycopene from carbon dioxide by cyanobacteria.

Start-Stop Assembly: a functionally scarless DNA assembly system optimized for metabolic engineering (2018)
Journal Article

© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research. DNA assembly allows individual DNA constructs or libraries to be assembled quickly and reliably. Most methods are either: (i) Modular, easily scalable and... Read More about Start-Stop Assembly: a functionally scarless DNA assembly system optimized for metabolic engineering.

Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824 (2016)
Journal Article

Background Clostridium acetobutylicum represents a paradigm chassis for the industrial production of the biofuel biobutanol and a focus for metabolic engineering. We have previously developed procedures for the creation of in-frame, marker-less de... Read More about Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824.