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A primer to directed evolution: current methodologies and future directions

Sellés Vidal, Lara; Isalan, Mark; Heap, John T.; Ledesma-Amaro, Rodrigo

Authors

Lara Sellés Vidal

Mark Isalan

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JOHN HEAP JOHN.HEAP@NOTTINGHAM.AC.UK
Associate Professor

Rodrigo Ledesma-Amaro



Abstract

Directed evolution is one of the most powerful tools for protein engineering and functions by harnessing natural evolution, but on a shorter timescale. It enables the rapid selection of variants of biomolecules with properties that make them more suitable for specific applications. Since the first in vitro evolution experiments performed by Sol Spiegelman in 1967, a wide range of techniques have been developed to tackle the main two steps of directed evolution: genetic diversification (library generation), and isolation of the variants of interest. This review covers the main modern methodologies, discussing the advantages and drawbacks of each, and hence the considerations for designing directed evolution experiments. Furthermore, the most recent developments are discussed, showing how advances in the handling of ever larger library sizes are enabling new research questions to be tackled.

Citation

Sellés Vidal, L., Isalan, M., Heap, J. T., & Ledesma-Amaro, R. (2023). A primer to directed evolution: current methodologies and future directions. RSC Chemical Biology, https://doi.org/10.1039/d2cb00231k

Journal Article Type Article
Acceptance Date Jan 18, 2023
Online Publication Date Jan 27, 2023
Publication Date Jan 27, 2023
Deposit Date Feb 20, 2023
Publicly Available Date Feb 20, 2023
Journal RSC Chemical Biology
Electronic ISSN 2633-0679
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
DOI https://doi.org/10.1039/d2cb00231k
Keywords Biochemistry, Genetics and Molecular Biology (miscellaneous); Molecular Biology; Biochemistry; Chemistry (miscellaneous)
Public URL https://nottingham-repository.worktribe.com/output/16790055
Publisher URL https://pubs.rsc.org/en/journals/journalissues/cb#!recentarticles&adv

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