A combinatorial DNA assembly approach to biosynthesis of N-linked glycans in E. coli

Passmore, Ian J.; Faulds-Pain, Alexandra; Abouelhadid, Sherif; Harrison, Mark A.; Hall, Catherine L.; Hitchen, Paul; Dell, Anne; Heap, John T.; Wren, Brendan W.

doi:10.1093/glycob/cwac082

All Outputs (3)

Developing a genetic engineering method for Acetobacterium wieringae to expand one-carbon valorization pathways (2023)
Journal Article

Background: Developing new bioprocesses to produce chemicals and fuels with reduced production costs will greatly facilitate the replacement of fossil-based raw materials. In most fermentation bioprocesses, the feedstock usually represents the highes... Read More about Developing a genetic engineering method for Acetobacterium wieringae to expand one-carbon valorization pathways.

A primer to directed evolution: current methodologies and future directions (2023)
Journal Article

Directed evolution is one of the most powerful tools for protein engineering and functions by harnessing natural evolution, but on a shorter timescale. It enables the rapid selection of variants of biomolecules with properties that make them more sui... Read More about A primer to directed evolution: current methodologies and future directions.

A combinatorial DNA assembly approach to biosynthesis of N-linked glycans in E. coli (2023)
Journal Article

Glycoengineering of recombinant glycans and glycoconjugates is a rapidly evolving field. However, the production and exploitation of glycans has lagged behind that of proteins and nucleic acids. Biosynthetic glycoconjugate production requires the coo... Read More about A combinatorial DNA assembly approach to biosynthesis of N-linked glycans in E. coli.