All Outputs (17)

Activated tissue resident memory T-cells (CD8+CD103+CD39+) uniquely predict survival in left sided “immune-hot” colorectal cancers (2023)
Journal Article
Talhouni, S., Fadhil, W., Mongan, N. P., Field, L., Hunter, K., Makhsous, S., …Ramage, J. M. (2023). Activated tissue resident memory T-cells (CD8+CD103+CD39+) uniquely predict survival in left sided “immune-hot” colorectal cancers. Frontiers in Immunology, 14, Article 1057292. https://doi.org/10.3389/fimmu.2023.1057292

Introduction: Characterization of the tumour immune infiltrate (notably CD8+ T-cells) has strong predictive survival value for cancer patients. Quantification of CD8 T-cells alone cannot determine antigenic experience, as not all infiltrating T-cells... Read More about Activated tissue resident memory T-cells (CD8+CD103+CD39+) uniquely predict survival in left sided “immune-hot” colorectal cancers.

The active form of Helicobacter pylori vacuolating cytotoxin induces decay-accelerating factor CD55 in association with intestinal metaplasia in the human gastric mucosa (2022)
Journal Article
Kaneko, K., Zaitoun, A. M., Letley, D. P., Rhead, J. L., Torres, J., Spendlove, I., …Robinson, K. (2022). The active form of Helicobacter pylori vacuolating cytotoxin induces decay-accelerating factor CD55 in association with intestinal metaplasia in the human gastric mucosa. Journal of Pathology, https://doi.org/10.1002/path.5990

High-level expression of decay-accelerating factor, CD55, has previously been found in human gastric cancer (GC) and intestinal metaplasia (IM) tissues. Therapeutic effects of CD55 inhibition in cancer have been reported. However, the role of Helicob... Read More about The active form of Helicobacter pylori vacuolating cytotoxin induces decay-accelerating factor CD55 in association with intestinal metaplasia in the human gastric mucosa.

Molecular and structural basis for Lewis glycan recognition by a cancer-targeting antibody (2020)
Journal Article
Soliman, C., Guy, A. J., Chua, J. X., Vankemmelbeke, M., McIntosh, R. S., Eastwood, S., …Ramsland, P. A. (2020). Molecular and structural basis for Lewis glycan recognition by a cancer-targeting antibody. Biochemical Journal, 477(17), 3219-3235. https://doi.org/10.1042/bcj20200454

Immunotherapy has been successful in treating many tumour types. The development of additional tumour-antigen binding monoclonal antibodies (mAbs) will help expand the range of immunotherapeutic targets. Lewis histo-blood group and related glycans ar... Read More about Molecular and structural basis for Lewis glycan recognition by a cancer-targeting antibody.

Increased IL-2 and reduced tgf-β upon t-cell stimulation are associated with GM-CSF upregulation in multiple immune cell types in multiple sclerosis (2020)
Journal Article
Aram, J., Frakich, N., Morandi, E., Alrouji, M., Samaraweera, A., Onion, D., …Constantinescu, C. S. (2020). Increased IL-2 and reduced tgf-β upon t-cell stimulation are associated with GM-CSF upregulation in multiple immune cell types in multiple sclerosis. Biomedicines, 8(7), Article 226. https://doi.org/10.3390/BIOMEDICINES8070226

© 2020 by the authors. Granulocyte macrophage colony stimulating factor (GM-CSF) is a pro-inflammatory cytokine produced by immune cells. Recent evidence suggests that GM-CSF plays an important role in multiple sclerosis (MS) pathogenesis. We investi... Read More about Increased IL-2 and reduced tgf-β upon t-cell stimulation are associated with GM-CSF upregulation in multiple immune cell types in multiple sclerosis.

Engineering the Human Fc Region Enables Direct Cell Killing by Cancer Glycan-Targeting Antibodies without the Need for Immune Effector Cells or Complement (2020)
Journal Article
Vankemmelbeke, M., McIntosh, R. S., Chua, J. X., Kirk, T., Daniels, I., Patsalidou, M., …Durrant, L. G. (2020). Engineering the Human Fc Region Enables Direct Cell Killing by Cancer Glycan-Targeting Antibodies without the Need for Immune Effector Cells or Complement. Cancer Research, 80(16), 3399-3412. https://doi.org/10.1158/0008-5472.CAN-19-3599

©2020 American Association for Cancer Research. Murine IgG3 glycan-targeting mAb often induces direct cell killing in the absence of immune effector cells or complement via a proinflammatory mechanism resembling oncotic necrosis. This cancer cell kil... Read More about Engineering the Human Fc Region Enables Direct Cell Killing by Cancer Glycan-Targeting Antibodies without the Need for Immune Effector Cells or Complement.

The terminal sialic acid of stage-specific embryonic antigen-4 has a crucial role in binding to a cancer-targeting antibody (2019)
Journal Article
Soliman, C., Chua, J. X., Vankemmelbeke, M., McIntosh, R. S., Guy, A. J., Spendlove, I., …Ramsland, P. A. (2020). The terminal sialic acid of stage-specific embryonic antigen-4 has a crucial role in binding to a cancer-targeting antibody. Journal of Biological Chemistry, 295(4), 1009-1020. https://doi.org/10.1074/jbc.ra119.011518

Cancer remains a leading cause of morbidity and mortality worldwide, requiring ongoing development of targeted therapeutics such as monoclonal antibodies. Carbohydrates on embryonic cells are often highly expressed in cancer and are therefore attract... Read More about The terminal sialic acid of stage-specific embryonic antigen-4 has a crucial role in binding to a cancer-targeting antibody.

Molecular expression patterns in the synovium and their association with advanced symptomatic knee osteoarthritis (2018)
Journal Article
Wyatt, L. A., Nwosu, L. N., Wilson, D., Hill, R., Spendlove, I., Bennett, A. J., …Walsh, D. A. (2018). Molecular expression patterns in the synovium and their association with advanced symptomatic knee osteoarthritis. Osteoarthritis and Cartilage, 27(4), 667-675. https://doi.org/10.1016/j.joca.2018.12.012

Objective: Osteoarthritis (OA) is a major source of knee pain. Mechanisms of OA knee pain are incompletely understood but include synovial pathology. We aimed to identify molecular expression patterns in the synovium associated with symptomatic knee... Read More about Molecular expression patterns in the synovium and their association with advanced symptomatic knee osteoarthritis.

Connexin 43 is an independent predictor of patient outcome in breast cancer patients (2018)
Journal Article
Chasampalioti, M., Green, A. R., Ellis, I. O., Rakha, E. A., Jackson, A. M., Spendlove, I., & Ramage, J. M. (2019). Connexin 43 is an independent predictor of patient outcome in breast cancer patients. Breast Cancer Research and Treatment, 174(1), 93-102. https://doi.org/10.1007/s10549-018-5063-9

Purpose

Gap junctions are specialized membrane structures that form channels between adjacent cells allowing cell communication. Gap junctions and specifically Connexin 43 (Cx43) are down-regulated in cancer; however, there are contrasting reports... Read More about Connexin 43 is an independent predictor of patient outcome in breast cancer patients.

A HIF-LIMD1 negative feedback mechanism mitigates the pro-tumorigenic effects of hypoxia (2018)
Journal Article
Foxler, D. E., Bridge, K. S., Foster, J. G., Grevitt, P., Curry, S., Shah, K. M., Davidson, K. M., Nagano, A., Gadaleta, E., Rhys, H. I., Kennedy, P. T., Hermida, M. A., Chang, T.-Y., Shaw, P. E., Reynolds, L. E., McKay, T. R., Wang, H.-. W., Ribeiro, P. S., Plevin, M. J., Lagos, D., …Sharp, T. V. (in press). A HIF-LIMD1 negative feedback mechanism mitigates the pro-tumorigenic effects of hypoxia. EMBO Molecular Medicine, Article e8304. https://doi.org/10.15252/emmm.201708304

The adaptive cellular response to low oxygen tensions is mediated by the hypoxia inducible factors (HIFs), a family of heterodimeric transcription factors composed of HIF-α and β subunits. Prolonged HIF expression is a key contributor to cellular t... Read More about A HIF-LIMD1 negative feedback mechanism mitigates the pro-tumorigenic effects of hypoxia.

High mobility group protein B1 is a predictor of poor survival in ovarian cancer (2017)
Journal Article
Machado, L. R., Moseley, P. M., Moss, R., Deen, S., Nolan, C. C., Spendlove, I., …Durrant, L. (2017). High mobility group protein B1 is a predictor of poor survival in ovarian cancer. Oncotarget, https://doi.org/10.18632/oncotarget.20538

High-mobility group protein B1 (HMGB1) has been implicated in numerous tumour types where expression regulates tumour cell growth and survival. We hypothesised that high HMGB1 expression in ovarian tumours would predict poor patient survival.
Using... Read More about High mobility group protein B1 is a predictor of poor survival in ovarian cancer.

A prebiotic galactooligosaccharide mixture reduces severity of hyperpnoea-induced bronchoconstriction and markers of airway inflammation (2016)
Journal Article
Williams, N. C., Johnson, M. A., Shaw, D. E., Spendlove, I., Vulevic, J., Sharpe, G. R., & Hunter, K. A. (2016). A prebiotic galactooligosaccharide mixture reduces severity of hyperpnoea-induced bronchoconstriction and markers of airway inflammation. British Journal of Nutrition, 116(5), 798-804. https://doi.org/10.1017/S0007114516002762

Gut microbes have a substantial influence on systemic immune function and allergic sensitisation. Manipulation of the gut microbiome through prebiotics may provide a potential strategy to influence the immunopathology of asthma. This study investigat... Read More about A prebiotic galactooligosaccharide mixture reduces severity of hyperpnoea-induced bronchoconstriction and markers of airway inflammation.

Stromal fibroblasts support dendritic cells to maintain IL-23/Th17 responses after exposure to ionizing radiation (2016)
Journal Article
Malecka, A., Wang, O., Shah, S., Sutavani, R. V., Spendlove, I., Ramage, J. M., …Jackson, A. M. (2016). Stromal fibroblasts support dendritic cells to maintain IL-23/Th17 responses after exposure to ionizing radiation. Journal of Leukocyte Biology, 100(2), 381-389. https://doi.org/10.1189/jlb.3A1015-474R

Dendritic cell function is modulated by stromal cells, including fibroblasts. Although poorly understood, the signals delivered through this crosstalk substantially alter dendritic cell biology. This is well illustrated with release of TNF-0/IL-113 f... Read More about Stromal fibroblasts support dendritic cells to maintain IL-23/Th17 responses after exposure to ionizing radiation.

Monoclonal antibodies targeting LecLex-related glycans with potent antitumor activity (2015)
Journal Article
Chua, J., Vankemmelbeke, M., McIntosh, R. S., Clarke, P. A., Moss, R., Parsons, T., Spendlove, I., Zaitoun, A. M., Madhusudan, S., & Durrant, L. (2015). Monoclonal antibodies targeting LecLex-related glycans with potent antitumor activity. Clinical Cancer Research, 21(13), 2963-2974. https://doi.org/10.1158/1078-0432.CCR-14-3030

© 2015 American Association for Cancer Research. Purpose: To produce antitumor monoclonal antibodies (mAbs) targeting glycans as they are aberrantly expressed in tumors and are coaccessory molecules for key survival pathways. Experimental Design: Two... Read More about Monoclonal antibodies targeting LecLex-related glycans with potent antitumor activity.

Structural biology of antibody recognition of carbohydrate epitopes and potential uses for targeted cancer immunotherapies (2015)
Journal Article

Monoclonal antibodies represent the most successful class of biopharmaceuticals for the treatment of cancer. Mechanisms of action of therapeutic antibodies are very diverse and reflect their ability to engage in antibody-dependent effector mechanisms... Read More about Structural biology of antibody recognition of carbohydrate epitopes and potential uses for targeted cancer immunotherapies.

Simulating the dynamics of T cell subsets throughout the lifetime (2011)
Book Chapter
Foan, S., Jackson, A. M., Spendlove, I., & Aickelin, U. (2011). Simulating the dynamics of T cell subsets throughout the lifetime. In P. Liò, G. Nicosia, & T. Stibor (Eds.), Artificial immune systems: 10th international conference, ICARIS 2011, Cambridge, UK, July 18-21, 2011: proceedings. Springer

It is widely accepted that the immune system undergoes age-related changes correlating with increased disease in the elderly. T cell subsets have been implicated. The aim of this work is firstly to implement and validate a simulation
of T regulatory... Read More about Simulating the dynamics of T cell subsets throughout the lifetime.

Total loss of MHC class I is an independent indicator of good prognosis in breast cancer (2005)
Journal Article
Madjd, Z., Spendlove, I., Pinder, S. E., Ellis, I. O., & Durrant, L. G. (2005). Total loss of MHC class I is an independent indicator of good prognosis in breast cancer. International Journal of Cancer, 117(2), https://doi.org/10.1002/ijc.21163

Tumours can be recognised by CTL and NK cells. CTL recognition depends on expression of MHC Class I loaded with peptides from tumour antigens. In contrast, loss of MHC Class I results in NK activation. In our study a large set of samples from patient... Read More about Total loss of MHC class I is an independent indicator of good prognosis in breast cancer.

Exploiting the Plasticity of Primary and Secondary Response Mechanisms in Artificial Immune Systems
Presentation / Conference Contribution
Greensmith, J., Jackson, A. M., & Spendlove, I. (2016, July). Exploiting the Plasticity of Primary and Secondary Response Mechanisms in Artificial Immune Systems. Presented at GECCO '16 Companion 2016 on Genetic and Evolutionary Computation Conference Companion, Denver, Colorado, USA

One of the key properties of the human immune system is to detect the presence of pathogens, and as such there are numberous immune algorithm which perform anomaly detection and pattern recognition. An additional facet of the human immune system is t... Read More about Exploiting the Plasticity of Primary and Secondary Response Mechanisms in Artificial Immune Systems.