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Engineering functional epithelium for regenerative medicine and in vitro organ models: A review (2013)
Journal Article
Vrana, N. E., Lavalle, P., Dokmeci, M. R., Dehghani, F., Ghaemmaghami, A. M., & Khademhosseini, A. (2013). Engineering functional epithelium for regenerative medicine and in vitro organ models: A review. Tissue Engineering Part B: Reviews, 19(6), 529-543. https://doi.org/10.1089/ten.teb.2012.0603

Recent advances in the fields of microfabrication, biomaterials, and tissue engineering have provided new opportunities for developing biomimetic and functional tissues with potential applications in disease modeling, drug discovery, and replacing da... Read More about Engineering functional epithelium for regenerative medicine and in vitro organ models: A review.

The development of a 3D immunocompetent model of human skin (2013)
Journal Article
Chau, D. Y., Johnson, C., MacNeil, S., Haycock, J. W., & Ghaemmaghami, A. M. (2013). The development of a 3D immunocompetent model of human skin. Biofabrication, 5(3), Article 035011. https://doi.org/10.1088/1758-5082/5/3/035011

As the first line of defence, skin is regularly exposed to a variety of biological, physical and chemical insults. Therefore, determining the skin sensitization potential of new chemicals is of paramount importance from the safety assessment and regu... Read More about The development of a 3D immunocompetent model of human skin.

Label-free separation and sorting of human monocytes and T-cells by electrowetting and dielectrophoresis (2013)
Presentation / Conference Contribution
Huang, C. Y., Chiang, M. Y., Fan, S. K., Ghaemmaghami, A. M., & Hsu, W. (2013). Label-free separation and sorting of human monocytes and T-cells by electrowetting and dielectrophoresis. In 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (873-876). https://doi.org/10.1109/NEMS.2013.6559863

We report a label-free separation and sorting approach based on electrowetting-on-dielectric (EWOD) and dielectrophoresis (DEP) to manipulate human monocyte (THP-1) and T-cell (Jurkat) lines in droplets without any impact on cell viability. In additi... Read More about Label-free separation and sorting of human monocytes and T-cells by electrowetting and dielectrophoresis.

The role of lectins in allergic sensitization and allergic disease (2013)
Journal Article
Salazar, F., Sewell, H. F., Shakib, F., & Ghaemmaghami, A. M. (2013). The role of lectins in allergic sensitization and allergic disease. Journal of Allergy and Clinical Immunology, 132(1), 27-36. https://doi.org/10.1016/j.jaci.2013.02.001

Allergic diseases are a global public health issue affecting millions of persons around the world. However, full understanding of the molecular basis of this group of chronic inflammatory disorders remains rather elusive. Recently, the role of carboh... Read More about The role of lectins in allergic sensitization and allergic disease.

Electrospun PLGA fibre sheets incorporating fluorescent nanosensors: Self-reporting scaffolds for application in tissue engineering (2012)
Journal Article
Harrington, H. C., Rose, F. R., Reinwald, Y., Buttery, L. D., Ghaemmaghami, A. M., & Aylott, J. W. (2013). Electrospun PLGA fibre sheets incorporating fluorescent nanosensors: Self-reporting scaffolds for application in tissue engineering. Analytical Methods, 5(1), 68-71. https://doi.org/10.1039/c2ay25771h

Ratiometric analyte responsive nanosensors have been incorporated into electrospun poly(lactic-co-glycolic) acid (PLGA) fibres to create self-reporting scaffolds. It has been demonstrated that the self-reporting scaffolds could be utilised to monitor... Read More about Electrospun PLGA fibre sheets incorporating fluorescent nanosensors: Self-reporting scaffolds for application in tissue engineering.

Tissue transglutaminase (TG-2) modified amniotic membrane: A novel scaffold for biomedical applications (2012)
Journal Article
Chau, D. Y., Brown, S. V., Mather, M. L., Hutter, V., Tint, N. L., Dua, H. S., …Ghaemmaghami, A. M. (2012). Tissue transglutaminase (TG-2) modified amniotic membrane: A novel scaffold for biomedical applications. Biomedical Materials, 7(4), Article 045011. https://doi.org/10.1088/1748-6041/7/4/045011

The amniotic membrane (AM) is considered as a natural cell culture substrate and has occasionally been exploited in regenerative medicine especially for ocular surface reconstruction and dermal wound healing applications. However, its use is limited... Read More about Tissue transglutaminase (TG-2) modified amniotic membrane: A novel scaffold for biomedical applications.

Tissue transglutaminase treatment leads to concentration-dependent changes in dendritic cell phenotype - implications for the role of transglutaminase in coeliac disease (2012)
Journal Article
Dalleywater, W. J., Chau, D. Y., & Ghaemmaghami, A. M. (2012). Tissue transglutaminase treatment leads to concentration-dependent changes in dendritic cell phenotype - implications for the role of transglutaminase in coeliac disease. BMC Immunology, 13, Article 20. https://doi.org/10.1186/1471-2172-13-20

Dendritic cells (DCs) are part of the innate immune system with a key role in initiating and modulating T cell mediated immune responses. Coeliac disease is caused by inappropriate activation of such a response leading to small intestinal inflammatio... Read More about Tissue transglutaminase treatment leads to concentration-dependent changes in dendritic cell phenotype - implications for the role of transglutaminase in coeliac disease.

Separation of dendritic and T cells using electrowetting and dielectrophoresis (2012)
Presentation / Conference Contribution
Chen, C. A., Chen, C. H., Ghaemmaghami, A. M., & Fan, S. K. (2012). Separation of dendritic and T cells using electrowetting and dielectrophoresis. In 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS) (183-186). https://doi.org/10.1109/NEMS.2012.6196752

The research of immune cells is fundamental to many biological studies. Dendritic cells have the ability to induce a primary immune response in resting naive T cells. The aim of this work is to separate the activated T cells from dendritic cells on a... Read More about Separation of dendritic and T cells using electrowetting and dielectrophoresis.

Biomimetic tissues on a chip for drug discovery (2011)
Journal Article
Ghaemmaghami, A. M., Hancock, M. J., Harrington, H., Kaji, H., & Khademhosseini, A. (2012). Biomimetic tissues on a chip for drug discovery. Drug Discovery Today, 17(3-4), 173-181. https://doi.org/10.1016/j.drudis.2011.10.029

Developing biologically relevant models of human tissues and organs is an important enabling step for disease modeling and drug discovery. Recent advances in tissue engineering, biomaterials and microfluidics have led to the development of microscale... Read More about Biomimetic tissues on a chip for drug discovery.

Uniform cell colonization of porous 3-D scaffolds achieved using radial control of surface chemistry (2011)
Journal Article
Intranuovo, F., Howard, D., White, L. J., Johal, R. K., Ghaemmaghami, A. M., Favia, P., …Alexander, M. R. (2011). Uniform cell colonization of porous 3-D scaffolds achieved using radial control of surface chemistry. Acta Biomaterialia, 7(9), 3336-3344. https://doi.org/10.1016/j.actbio.2011.05.020

Uniform cellular distribution is a prerequisite to forming tissue within porous scaffolds, but the seeding process often results in preferential adhesion of cells at the periphery. We develop a vapour phase coating strategy which is readily applicabl... Read More about Uniform cell colonization of porous 3-D scaffolds achieved using radial control of surface chemistry.

Glycosylation of surface Ig creates a functional bridge between human follicular lymphoma and microenvironmental lectins (2010)
Journal Article
Coelho, V., Krysov, S., Ghaemmaghami, A. M., Emara, M., Potter, K. N., Johnson, P., …Stevenson, F. K. (2010). Glycosylation of surface Ig creates a functional bridge between human follicular lymphoma and microenvironmental lectins. Proceedings of the National Academy of Sciences, 107(43), 18587-18592. https://doi.org/10.1073/pnas.1009388107

Surface Ig (sIg) of follicular lymphoma (FL) is vital for tumor cell survival. We found previously that the Ig in FL is unusual, because the variable region genes carry sequence motifs for N-glycan addition. These are introduced by somatic mutation a... Read More about Glycosylation of surface Ig creates a functional bridge between human follicular lymphoma and microenvironmental lectins.

Label-free molecular imaging of immunological synapses between dendritic and T cells by Raman micro-spectroscopy (2010)
Presentation / Conference Contribution
Zoladek, A. B., Johal, R. K., Garcia-Nieto, S., Pascut, F., Shakesheff, K. M., Ghaemmaghami, A. M., & Notingher, I. (2010, June). Label-free molecular imaging of immunological synapses between dendritic and T cells by Raman micro-spectroscopy. Presented at SPEC 2010 Shedding Light on Disease: Optical Diagnosis for the New Millennium, Manchester, UK

Confocal Raman micro-spectroscopy (CRMS) was used to measure spectral images of immunological synapse formation between dendritic and T cells without using molecular labels or other invasive procedures. The purpose-built inverted CRMS instrument inte... Read More about Label-free molecular imaging of immunological synapses between dendritic and T cells by Raman micro-spectroscopy.

The mannose receptor mediates the uptake of diverse native allergens by dendritic cells and determines allergen-induced T cell polarization through modulation of IDO Activity (2010)
Journal Article
Royer, P. J., Emara, M., Yang, C., Al-Ghouleh, A., Tighe, P., Jones, N., …Ghaemmaghami, A. M. (2010). The mannose receptor mediates the uptake of diverse native allergens by dendritic cells and determines allergen-induced T cell polarization through modulation of IDO Activity. Journal of Immunology, 185(3), 1522-1531. https://doi.org/10.4049/jimmunol.1000774

The mannose receptor (MR) is a C-type lectin expressed by dendritic cells (DCs). We have investigated the ability of MR to recognize glycosylated allergens. Using a gene silencing strategy, we have specifically inhibited the expression of MR on human... Read More about The mannose receptor mediates the uptake of diverse native allergens by dendritic cells and determines allergen-induced T cell polarization through modulation of IDO Activity.

Differential protein expression by dendritic cells from atopic and non-atopic individuals after stimulation by the major house dust mite allergen der p 1 (2009)
Journal Article
Horlock, C., Shakib, F., Jones, N. S., Sewell, H. F., & Ghaemmaghami, A. M. (2009). Differential protein expression by dendritic cells from atopic and non-atopic individuals after stimulation by the major house dust mite allergen der p 1. International Archives of Allergy and Immunology, 150(3), 237-251. https://doi.org/10.1159/000222676

Background: Dendritic cells (DCs) are sentinels of the immune system and are known to play a key role in allergic responses. However, it is not clear how DCs that have been exposed to an allergen support Th2 type immune responses. It is possible that... Read More about Differential protein expression by dendritic cells from atopic and non-atopic individuals after stimulation by the major house dust mite allergen der p 1.

The molecular basis of allergenicity (2008)
Journal Article
Shakib, F., Ghaemmaghami, A. M., & Sewell, H. F. (2008). The molecular basis of allergenicity. Trends in Immunology, 29(12), 633-642. https://doi.org/10.1016/j.it.2008.08.007

Allergens are mostly innocuous antigens that elicit powerful T helper cell type 2 (Th2) responses leading to hyper-immunoglobulin E (IgE) production and allergy. Research carried out over several years has highlighted the possible role of the inheren... Read More about The molecular basis of allergenicity.

Allergen-driven suppression of thiol production by human dendritic cells and the effect of thiols on T cell function (2008)
Journal Article
Hasan, A. A., Ghaemmaghami, A. M., Fairclough, L., Robins, A., Sewell, H. F., & Shakib, F. (2009). Allergen-driven suppression of thiol production by human dendritic cells and the effect of thiols on T cell function. Immunobiology, 214(1), 2-16. https://doi.org/10.1016/j.imbio.2008.04.006

Dendritic cells are a major source of extracellular thiols needed for T cell activation, a process in which CD40-mediated stimulation plays a pivotal role. The Dermatophagoides pteronyssinus group 1 mite allergen (Der p 1) has previously been shown t... Read More about Allergen-driven suppression of thiol production by human dendritic cells and the effect of thiols on T cell function.

An immunoglobulin E-reactive chimeric human immunoglobulin G1 anti-idiotype inhibits basophil degranulation through cross-linking of FcεRI with FcγRIIb (2007)
Journal Article
Wigginton, S. J., Furtado, P. B., Armour, K. L., Clark, M. R., Robins, A., Emara, M., …Shakib, F. (2008). An immunoglobulin E-reactive chimeric human immunoglobulin G1 anti-idiotype inhibits basophil degranulation through cross-linking of FcεRI with FcγRIIb. Clinical and Experimental Allergy, 38(2), 313-319. https://doi.org/10.1111/j.1365-2222.2007.02896.x

Background: IgE binds to mast cells and basophils via its high-affinity receptor, FcεRI, and cross-linking of FcεRI-bound IgE molecules by allergen leads to the release of allergic mediators characteristic of type I hypersensitivity reactions. Previo... Read More about An immunoglobulin E-reactive chimeric human immunoglobulin G1 anti-idiotype inhibits basophil degranulation through cross-linking of FcεRI with FcγRIIb.

Elevated CD16 expression by monocytes from patients with tumor necrosis factor receptor-associated periodic syndrome (2007)
Journal Article
Todd, I., Radford, P. M., Ziegler-Heitbrock, L., Ghaemmaghami, A. M., Powell, R. J., & Tighe, P. J. (2007). Elevated CD16 expression by monocytes from patients with tumor necrosis factor receptor-associated periodic syndrome. Arthritis and Rheumatism, 56(12), 4182-4188. https://doi.org/10.1002/art.23133

Objective. Tumor necrosis factor receptor-associated periodic syndrome (TRAPS) is an inherited autosomal-dominant autoinflammatory condition caused by mutations in the ectodomain of the 55-kd tumor necrosis factor (TNF) receptor superfamily 1A. Proin... Read More about Elevated CD16 expression by monocytes from patients with tumor necrosis factor receptor-associated periodic syndrome.

The detection of ADAM8 protein on cells of the human immune system and the demonstration of its expression on peripheral blood B cells, dendritic cells and monocyte subsets (2007)
Journal Article
Richens, J., Fairclough, L., Ghaemmaghami, A. M., Mahdavi, J., Shakib, F., & Sewell, H. F. (2007). The detection of ADAM8 protein on cells of the human immune system and the demonstration of its expression on peripheral blood B cells, dendritic cells and monocyte subsets. Immunobiology, 212(1), 29-38. https://doi.org/10.1016/j.imbio.2006.06.012

A disintegrin and metalloprotease (ADAM) proteins have wide ranging functions, including proteolytic cleavage of cell surface molecules, cell fusion, cell adhesion and intracellular signalling. Recent evidence suggests the involvement of ADAM8 in all... Read More about The detection of ADAM8 protein on cells of the human immune system and the demonstration of its expression on peripheral blood B cells, dendritic cells and monocyte subsets.