The mannose receptor mediates the uptake of diverse native allergens by dendritic cells and determines allergen-induced T cell polarization through modulation of IDO Activity

Royer, Pierre Joseph; Emara, Mohamed; Yang, Chaoxing; Al-Ghouleh, Abeer; Tighe, Paddy; Jones, Nick; Sewell, Herb F.; Shakib, Farouk; Martinez-Pomares, Luisa; Ghaemmaghami, Amir M.

Abstract

The mannose receptor (MR) is a C-type lectin expressed by dendritic cells (DCs). We have investigated the ability of MR to recognize glycosylated allergens. Using a gene silencing strategy, we have specifically inhibited the expression of MR on human monocyte-derived DCs. We show that MR mediates internalization of diverse allergens from mite (Der p 1 and Der p 2), dog (Can f 1), cockroach (Bla g 2), and peanut (Ara h 1) through their carbohydrate moieties. All of these allergens bind to the C-type lectin-like carbohydrate recognition domains 4-7 of MR. We have also assessed the contribution of MR to T cell polarization after allergen exposure. We show that silencing MR expression on monocyte-derived DCs reverses the Th2 cell polarization bias, driven by Der p 1 allergen exposure, through upregulation of IDO activity. In conclusion, our work demonstrates a major role for MR in glycoallergen recognition and in the development of Th2 responses. Copyright © 2010 by The American Association of Immunologists, Inc.

Citation

Royer, P. J., Emara, M., Yang, C., Al-Ghouleh, A., Tighe, P., Jones, N., Sewell, H. F., Shakib, F., Martinez-Pomares, L., & Ghaemmaghami, A. M. (2010). The mannose receptor mediates the uptake of diverse native allergens by dendritic cells and determines allergen-induced T cell polarization through modulation of IDO Activity. Journal of Immunology, 185(3), 1522-1531. https://doi.org/10.4049/jimmunol.1000774

• Journal Article Type: Article
• Acceptance Date: May 26, 2010
• Online Publication Date: Aug 1, 2010
• Publication Date: Aug 1, 2010
• Deposit Date: Jan 3, 2023
• Journal: Journal of Immunology
• Print ISSN: 0022-1767
• Electronic ISSN: 1550-6606
• Publisher: American Association of Immunologists
• Peer Reviewed: Peer Reviewed
• Volume: 185
• Issue: 3
• Pages: 1522-1531
• DOI: https://doi.org/10.4049/jimmunol.1000774
• Public URL: https://nottingham-repository.worktribe.com/output/3096460
• Publisher URL: https://journals.aai.org/jimmunol/article/185/3/1522/39103/The-Mannose-Receptor-Mediates-the-Uptake-of