Separation of dendritic and T cells using electrowetting and dielectrophoresis

Chen, Chang An; Chen, Chiun Hsun; Ghaemmaghami, Amir M.; Fan, Shih Kang

Abstract

The research of immune cells is fundamental to many biological studies. Dendritic cells have the ability to induce a primary immune response in resting naive T cells. The aim of this work is to separate the activated T cells from dendritic cells on a digital microfluidic device where droplets are driven by electrowetting-on-dielectric (EWOD). The cells suspended in EWOD-driven droplets are separated and concentrated using a high frequency electric signal which generates non-uniform electric fields and dielectrophoresis (DEP) forces exerting on dendritic cells and T cells. Separation and concentration of dendritic cells and T cells are demonstrated in a droplet using EWOD and DEP. © 2012 IEEE.

Citation

Chen, C. A., Chen, C. H., Ghaemmaghami, A. M., & Fan, S. K. (2012). Separation of dendritic and T cells using electrowetting and dielectrophoresis. In 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS) (183-186). https://doi.org/10.1109/NEMS.2012.6196752

• Presentation Conference Type: Edited Proceedings
• Conference Name: 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2012
• Start Date: Mar 5, 2012
• End Date: Mar 8, 2012
• Online Publication Date: May 10, 2012
• Publication Date: Mar 8, 2012
• Deposit Date: Jan 3, 2023
• Publisher: Institute of Electrical and Electronics Engineers
• Pages: 183-186
• Book Title: 2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)
• ISBN: 9781467311229
• DOI: https://doi.org/10.1109/NEMS.2012.6196752
• Public URL: https://nottingham-repository.worktribe.com/output/3097486
• Publisher URL: https://ieeexplore.ieee.org/document/6196752