All Outputs (125)

Superior T cell activation by ESAT-6 as compared with the ESAT-6-CFP-10 complex (2005)
Journal Article
Marei, A., Ghaemmaghami, A., Renshaw, P., Wiselka, M., Barer, M., Carr, M., & Ziegler-Heitbrock, L. (2005). Superior T cell activation by ESAT-6 as compared with the ESAT-6-CFP-10 complex. International Immunology, 17(11), 1439-1446. https://doi.org/10.1093/intimm/dxh322

Using intracellular cytokine staining we show herein that T cells will respond to short-term (6 h) activation with phorbol ester plus ionomycin by production of tumor necrosis factor (TNF), IFN-γ or both. Here CD4 T cells preferentially produce TNF a... Read More about Superior T cell activation by ESAT-6 as compared with the ESAT-6-CFP-10 complex.

Activity profile of dust mite allergen extract using substrate libraries and functional proteomic microarrays (2004)
Journal Article
Harris, J., Mason, D. E., Li, J., Burdick, K. W., Backes, B. J., Chen, T., …Winssinger, N. (2004). Activity profile of dust mite allergen extract using substrate libraries and functional proteomic microarrays. Cell Chemical Biology, 11(10), 1361-1372. https://doi.org/10.1016/j.chembiol.2004.08.008

Enzymatic activity in the fecal droppings from the house dust mite has been postulated to contribute to the elicited allergic response. Screening dust mite extracts through 137,180 tetrapeptide fluorogenic substrates allowed for the characterization... Read More about Activity profile of dust mite allergen extract using substrate libraries and functional proteomic microarrays.

The proteolytic activity of the major dust mite allergen Der p 1 conditions dendritic cells to produce less interleukin-12: Allergen-induced Th2 bias determined at the dendritic cell level (2002)
Journal Article
Ghaemmaghami, A. M., Gough, L., Sewell, H. F., & Shakib, F. (2002). The proteolytic activity of the major dust mite allergen Der p 1 conditions dendritic cells to produce less interleukin-12: Allergen-induced Th2 bias determined at the dendritic cell level. Clinical and Experimental Allergy, 32(10), 1468-1475. https://doi.org/10.1046/j.1365-2745.2002.01504.x

Background: The proteolytic activity of the house dust mite allergen Der p 1 has recently been shown to bias Th cell subset development in favour of Th2. Apart from its direct effect on T cells, it is conceivable that the proteolytic activity of Der... Read More about The proteolytic activity of the major dust mite allergen Der p 1 conditions dendritic cells to produce less interleukin-12: Allergen-induced Th2 bias determined at the dendritic cell level.

Human T cells that have been conditioned by the proteolytic activity of the major dust mite allergen Der p 1 trigger enhanced immunoglobulin E synthesis by B cells (2002)
Journal Article
Ghaemmaghami, A. M., & Shakib, F. (2002). Human T cells that have been conditioned by the proteolytic activity of the major dust mite allergen Der p 1 trigger enhanced immunoglobulin E synthesis by B cells. Clinical and Experimental Allergy, 32(5), 728-732. https://doi.org/10.1046/j.1365-2222.2002.01374.x

Background: We have previously demonstrated that the proteolytic activity of Der p 1 selectively cleaves human CD25, the 55 kDa α subunit of the IL-2 receptor. As a result of cleavage of surface CD25, peripheral blood T cells produce less IFN-γ and m... Read More about Human T cells that have been conditioned by the proteolytic activity of the major dust mite allergen Der p 1 trigger enhanced immunoglobulin E synthesis by B cells.

Human T cell subset commitment determined by the intrinsic property of antigen: The proteolytic activity of the major mite allergen Der p 1 conditions T cells to produce more IL-4 and less IFN-γ (2001)
Journal Article
Ghaemmaghami, A. M., Robins, A., Gough, L., Sewell, H. F., & Shakib, F. (2001). Human T cell subset commitment determined by the intrinsic property of antigen: The proteolytic activity of the major mite allergen Der p 1 conditions T cells to produce more IL-4 and less IFN-γ. European Journal of Immunology, 31(4), 1211-1216. https://doi.org/10.1002/1521-4141%28200104%2931%3A4%3C1211%3A%3AAID-IMMU1211%3E3.0.CO%3B2-R

The house dust mite Dermatophagoides pteronyssinus allergen Der p 1 elicits IgE antibody responses in a significant proportion of patients suffering from dust mite allergy. We have recently shown that Der p 1 proteolytically cleaves a cell surface mo... Read More about Human T cell subset commitment determined by the intrinsic property of antigen: The proteolytic activity of the major mite allergen Der p 1 conditions T cells to produce more IL-4 and less IFN-γ.