Human T cell subset commitment determined by the intrinsic property of antigen: The proteolytic activity of the major mite allergen Der p 1 conditions T cells to produce more IL-4 and less IFN-γ

Ghaemmaghami, Amir M.; Robins, Adrian; Gough, Lucy; Sewell, Herb F.; Shakib, Farouk

Abstract

The house dust mite Dermatophagoides pteronyssinus allergen Der p 1 elicits IgE antibody responses in a significant proportion of patients suffering from dust mite allergy. We have recently shown that Der p 1 proteolytically cleaves a cell surface molecule involved in the homeostatic control of human IgE synthesis, namely the IL-2 receptor (CD25) on T cells. As a result, these T cells show markedly diminished proliferation and IFN-γ secretion in response to stimulation by anti-CD3 antibody. However, these observations still leave open the important issue of whether CD25 cleavage, and the consequent suppression of IFN-γ secretion, leads to enhanced IL-4 secretion, and whether such cytokine changes would be exhibited by both CD4 and CD8 T cells. Here we demonstrate for the first time that the proteolytic activity of Der p 1 biases human CD4 and CD8 T cells towards a type 2 cytokine profile. Our data provide compelling evidence for the role of the proteolytic activity of Der p 1 in creating a microenvironment conductive for IgE synthesis.

Citation

Ghaemmaghami, A. M., Robins, A., Gough, L., Sewell, H. F., & Shakib, F. (2001). Human T cell subset commitment determined by the intrinsic property of antigen: The proteolytic activity of the major mite allergen Der p 1 conditions T cells to produce more IL-4 and less IFN-γ. European Journal of Immunology, 31(4), 1211-1216. https://doi.org/10.1002/1521-4141%28200104%2931%3A4%3C1211%3A%3AAID-IMMU1211%3E3.0.CO%3B2-R

• Journal Article Type: Article
• Acceptance Date: Jan 22, 2001
• Online Publication Date: Mar 29, 2001
• Publication Date: May 2, 2001
• Deposit Date: Jan 3, 2023
• Journal: European Journal of Immunology
• Print ISSN: 0014-2980
• Electronic ISSN: 1521-4141
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 31
• Issue: 4
• Pages: 1211-1216
• DOI: https://doi.org/10.1002/1521-4141%28200104%2931%3A4%3C1211%3A%3AAID-IMMU1211%3E3.0.CO%3B2-R
• Public URL: https://nottingham-repository.worktribe.com/output/3097660
• Publisher URL: https://onlinelibrary.wiley.com/doi/10.1002/1521-4141(200104)31:4%3C1211::AID-IMMU1211%3E3.0.CO;2-R