A novel analytical ultracentrifugation based approach to the low resolution structure of gum arabic

Gillis, Richard B.; Adams, Gary G.; Alzahrani, Qushmua; Harding, Stephen E.; Allen Bush, C.

Abstract

Under investigation are the structural properties of gum arabic, an industrially important biopolymer for use as a stabilizer or in drug delivery, using Analytical Ultracentrifugation—a well-established, matrix-free probe for macromolecular size and shape. These results are combined with chromatographically-coupled methods (multi-angle light scattering, differential press imbalance viscometry) to provide a global analysis of its structure in varying ionic strength conditions. This analysis indicates that gum Arabic may have a compact, elliptical structure in solution, the significance of which for biotechnological use is indicated. This modelling method can be applied to other biopolymers and synthetic polymers.

Citation

Gillis, R. B., Adams, G. G., Alzahrani, Q., Harding, S. E., & Allen Bush, C. (2016). A novel analytical ultracentrifugation based approach to the low resolution structure of gum arabic. Biopolymers, 105(9), 618-625. https://doi.org/10.1002/bip.22823

• Journal Article Type: Article
• Acceptance Date: Feb 12, 2016
• Online Publication Date: Jun 18, 2016
• Publication Date: Sep 1, 2016
• Deposit Date: Apr 3, 2017
• Publicly Available Date: Apr 3, 2017
• Journal: Biopolymers
• Print ISSN: 0006-3525
• Electronic ISSN: 1097-0282
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 105
• Issue: 9
• Pages: 618-625
• DOI: https://doi.org/10.1002/bip.22823
• Keywords: acacia; AUC; characterization; conformation; gum arabic; hydrodynamic; sedimentation velocity; ultracentrifugation
• Public URL: https://nottingham-repository.worktribe.com/output/975033
• Publisher URL: http://onlinelibrary.wiley.com/doi/10.1002/bip.22823/abstract
• Additional Information: This is the peer reviewed version of the following article: Gillis, R. B., Adams, G. G., Alzahrani, Q. and Harding, S. E. (2016), A novel analytical ultracentrifugation based approach to the low resolution structure of gum arabic. Biopolymers, 105: 618–625, which has been published in final form at http://dx.doi.org/10.1002/bip.22823. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
• Contract Date: Apr 3, 2017