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A novel analytical ultracentrifugation based approach to the low resolution structure of gum arabic

Gillis, Richard B.; Adams, Gary G.; Alzahrani, Qushmua; Harding, Stephen E.; Allen Bush, C.

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Authors

Richard B. Gillis

Gary G. Adams

Qushmua Alzahrani

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STEPHEN HARDING STEVE.HARDING@NOTTINGHAM.AC.UK
Professor of Applied Biochemistry

C. Allen Bush



Abstract

Under investigation are the structural properties of gum arabic, an industrially important biopolymer for use as a stabilizer or in drug delivery, using Analytical Ultracentrifugation—a well-established, matrix-free probe for macromolecular size and shape. These results are combined with chromatographically-coupled methods (multi-angle light scattering, differential press imbalance viscometry) to provide a global analysis of its structure in varying ionic strength conditions. This analysis indicates that gum Arabic may have a compact, elliptical structure in solution, the significance of which for biotechnological use is indicated. This modelling method can be applied to other biopolymers and synthetic polymers.

Citation

Gillis, R. B., Adams, G. G., Alzahrani, Q., Harding, S. E., & Allen Bush, C. (2016). A novel analytical ultracentrifugation based approach to the low resolution structure of gum arabic. Biopolymers, 105(9), 618-625. https://doi.org/10.1002/bip.22823

Journal Article Type Article
Acceptance Date Feb 12, 2016
Online Publication Date Jun 18, 2016
Publication Date Sep 1, 2016
Deposit Date Apr 3, 2017
Publicly Available Date Apr 3, 2017
Journal Biopolymers
Print ISSN 0006-3525
Electronic ISSN 1097-0282
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 105
Issue 9
Pages 618-625
DOI https://doi.org/10.1002/bip.22823
Keywords acacia; AUC; characterization; conformation; gum arabic; hydrodynamic; sedimentation velocity; ultracentrifugation
Public URL https://nottingham-repository.worktribe.com/output/975033
Publisher URL http://onlinelibrary.wiley.com/doi/10.1002/bip.22823/abstract
Additional Information This is the peer reviewed version of the following article: Gillis, R. B., Adams, G. G., Alzahrani, Q. and Harding, S. E. (2016), A novel analytical ultracentrifugation based approach to the low resolution structure of gum arabic. Biopolymers, 105: 618–625, which has been published in final form at http://dx.doi.org/10.1002/bip.22823. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

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