Detection of transmissible viral proventriculitis (TVP) and Chicken proventricular necrosis virus (CPNV) in the United Kingdom

Grau-Roma, Llorenc; Reid, Kirsty; de Brot, Simone; Jennison, Richard; Barrow, Paul; S�nchez, Ra�l; Nofrar�as, Miquel; Clark, Mike; Maj�, Nat�lia

Abstract

Increasing evidence suggests that a new birnavirus, named Chicken proventricular necrosis virus (CPNV), is the aetiological agent of transmissible viral proventriculitis (TVP). The present work aimed to explore the possible presence of both TVP and CPNV in the UK. Forty-four chickens showing TVP-compatible gross lesions were classified into 3 groups based on the histological lesions: i) TVP-affected chickens: lymphocytic infiltration and glandular necrosis (n=15); ii) lymphocytic proventriculitis (LP)-affected chickens: lymphocytic infiltration without necrosis (n=18); and iii) without proventriculitis (WP): no lymphocytic infiltration or necrosis (n=11). Nine proventriculi (7 out of 15 corresponding to TVP, and 2 out of 11 corresponding to LP) were positive for CPNV by RT-PCR. These results support the previously suggested idea of CPNV as causative agent of TVP. Moreover, this data shows that CPNV can also be detected in a number of cases with LP, which do not fulfil the histological TVP criteria. Phylogenetic analysis of partial sequences of gene VP1 showed that British CPNV sequences were closer to other European CPNV sequences and might constitute a different lineage from the American CPNV. TVP cases with negative CPNV PCR results may be due to chronic stages of the disease or to the reduced PCR sensitivity on formalin-fixed paraffin embedded tissues. However, involvement of other agents in some of the cases cannot totally be ruled out. As far as the authors are aware, this is the first peer-reviewed report of TVP as well as of CPNV in the UK, and the first exploratory CPNV phylogenetic study.

Citation

Grau-Roma, L., Reid, K., de Brot, S., Jennison, R., Barrow, P., Sánchez, R., …Majó, N. (2017). Detection of transmissible viral proventriculitis (TVP) and Chicken proventricular necrosis virus (CPNV) in the United Kingdom. Avian Pathology, 46(1), 68-75. https://doi.org/10.1080/03079457.2016.1207751

• Journal Article Type: Article
• Acceptance Date: Jun 23, 2016
• Online Publication Date: Dec 1, 2016
• Publication Date: Jan 1, 2017
• Deposit Date: Oct 26, 2016
• Publicly Available Date: Dec 1, 2016
• Journal: Avian Pathology
• Print ISSN: 0307-9457
• Electronic ISSN: 0307-9457
• Publisher: Taylor and Francis
• Peer Reviewed: Peer Reviewed
• Volume: 46
• Issue: 1
• Pages: 68-75
• DOI: https://doi.org/10.1080/03079457.2016.1207751
• Keywords: Birnavirus; chicken proventricular necrosis virus (CPNV); transmissible viral proventriculitis (TVP); natural infection; poultry
• Public URL: https://nottingham-repository.worktribe.com/output/971232
• Publisher URL: http://www.tandfonline.com/doi/abs/10.1080/03079457.2016.1207751
• Additional Information: This is an Accepted Manuscript of an article published by Taylor & Francis in Avian Pathology on 1 Dec 2016, available online: http://www.tandfonline.com/10.1080/03079457.2016.1207751.