Lee Hunt
Formation of the stomatal outer cuticular ledge requires a guard cell wall proline-rich protein
Hunt, Lee; Amsbury, Samuel; Baillie, Alice; Movahedi, Mahsa; Mitchell, Alice; Afsharinafar, Mana; Swarup, Kamal; Denyer, Thomas; Hobbs, Jamie K.; Swarup, Ranjan; Fleming, Andrew J.; Gray, Julie E.
Authors
Samuel Amsbury
Alice Baillie
Mahsa Movahedi
Alice Mitchell
Mana Afsharinafar
Kamal Swarup
Thomas Denyer
Jamie K. Hobbs
RANJAN SWARUP RANJAN.SWARUP@NOTTINGHAM.AC.UK
Associate Professor
Andrew J. Fleming
Julie E. Gray
Abstract
Stomata are formed by a pair of guard cells which have thickened, elastic cell walls to withstand the large increases in turgor pressure that have to be generated to open the pore that they surround. We have characterised FOCL1, a guard cell-expressed, secreted protein with homology to hydroxyproline-rich cell wall proteins. FOCL1-GFP localises to the guard cell outer cuticular ledge and plants lacking FOCL1 produce stomata without a cuticular ledge. Instead the majority of stomatal pores are entirely covered-over by a continuous fusion of the cuticle, and consequently plants have decreased levels of transpiration and display drought tolerance. The focl1 guard cells are larger and less able to reduce the aperture of their stomatal pore in response to closure signals suggesting that the flexibility of guard cell walls is impaired. FOCL1 is also expressed in lateral root initials where it aids lateral root emergence. We propose that FOCL1 acts in these highly specialised cells of the stomata and root to impart cell wall strength at high turgor and/or to facilitate interactions between the cell wall and the cuticle.
Citation
Hunt, L., Amsbury, S., Baillie, A., Movahedi, M., Mitchell, A., Afsharinafar, M., …Gray, J. E. (2017). Formation of the stomatal outer cuticular ledge requires a guard cell wall proline-rich protein. Plant Physiology, 174(2), https://doi.org/10.1104/pp.16.01715
Journal Article Type | Article |
---|---|
Acceptance Date | Jan 30, 2017 |
Online Publication Date | Feb 2, 2017 |
Publication Date | Aug 1, 2017 |
Deposit Date | Aug 15, 2017 |
Publicly Available Date | Aug 15, 2017 |
Journal | Plant Physiology |
Print ISSN | 0032-0889 |
Electronic ISSN | 1532-2548 |
Publisher | American Society of Plant Biologists |
Peer Reviewed | Peer Reviewed |
Volume | 174 |
Issue | 2 |
DOI | https://doi.org/10.1104/pp.16.01715 |
Public URL | https://nottingham-repository.worktribe.com/output/967286 |
Publisher URL | http://www.plantphysiol.org/content/174/2/689 |
Contract Date | Aug 15, 2017 |
Files
Plant Physiol.-2017-Hunt-pp.16.01715.pdf
(1.3 Mb)
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