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Quiescent center initiation in the Arabidopsis lateral root primordia is dependent on the SCARECROW transcription factor

Goh, Tatsuaki; Toyokura, Koichi; Wells, Darren M.; Swarup, Kamal; Yamamoto, Mayuko; Mimura, Tetsuro; Weijers, Dolf; Fukaki, Hidehiro; Laplaze, Laurent; Bennett, Malcolm J.; Guyomarc'h, Soazig


Tatsuaki Goh

Koichi Toyokura

Principal Research Fellow

Kamal Swarup

Mayuko Yamamoto

Tetsuro Mimura

Dolf Weijers

Hidehiro Fukaki

Laurent Laplaze

Soazig Guyomarc'h


Lateral root (LR) formation is an important determinant of root system architecture. In Arabidopsis, LRs originate from pericycle cells, which undergo a programme of morphogenesis to generate a new LR meristem. Despite its importance for root meristem organisation, the onset of organizing center (termed quiescent center; QC) formation during LR morphogenesis remains unclear. Here, we used live 3D confocal imaging to monitor cell organization and identity acquisition during LR development. Our dynamic observations revealed an early morphogenesis phase and a late meristem formation phase as proposed in the bi-phasic growth model described by Sussex and co-workers. LR QC establishment coincided with this developmental phase transition. QC precursor cells originated from the outer layer of stage II LR primordia, within which the SCARECROW (SCR) transcription factor was specifically expressed. Disrupting SCR function abolished periclinal divisions in this LR primordia cell layer and perturbed the formation of QC precursor cells. We conclude that de novo QC establishment in LR primordia operates via SCR-mediated formative cell division and coincides with the developmental phase transition.


Goh, T., Toyokura, K., Wells, D. M., Swarup, K., Yamamoto, M., Mimura, T., …Guyomarc'h, S. (2016). Quiescent center initiation in the Arabidopsis lateral root primordia is dependent on the SCARECROW transcription factor. Development, 143(18), 3363-3371.

Journal Article Type Article
Acceptance Date Jul 26, 2016
Online Publication Date Aug 10, 2016
Publication Date Sep 13, 2016
Deposit Date Oct 24, 2016
Publicly Available Date Oct 24, 2016
Journal Development
Print ISSN 0950-1991
Electronic ISSN 1477-9129
Publisher Company of Biologists
Peer Reviewed Peer Reviewed
Volume 143
Issue 18
Pages 3363-3371
Keywords Lateral root organogenesis, Quiescent center, SCARECROW, 3D live imaging, Arabidopsis thaliana
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