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Self-sustaining closed-loop multienzyme mediated conversion of amines into alcohols in continuous reactions

Contente, Martina L.; Paradisi, Francesca

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Authors

Martina L. Contente

Francesca Paradisi



Abstract

The synthesis of alcohols from amines starting material is an excellent, yet challenging, strategy for the preparation of pharmaceuticals and polymers. Here, we developed a versatile, self-sustaining closed-loop multienzymatic platform for the biocatalytic synthesis of a large range of non-commercially available products in continuous flow with excellent yields (80>99%), reaction times and optical purity of secondary alcohols (>99 e.e.). This process was also extended to the conversion of biogenic amines into high value alcohols, such as the powerful anti-oxidant hydroxytyrosol, and the synthesis of enantiopure 2-arylpropanols via dynamic kinetic resolution of commercially affordable racemic amines. The system exploits the in situ immobilisation of transaminases and redox enzymes which were combined to cater for a fully automated, ultra-efficient synthetic platform with cofactor recycling, in-line recovery of benign by-products and recirculation of the aqueous media containing the recycled cofactors in catalytic amount, which increases the efficiency of the system by over 20-fold.

Citation

Contente, M. L., & Paradisi, F. (2018). Self-sustaining closed-loop multienzyme mediated conversion of amines into alcohols in continuous reactions. Nature Catalysis, 1, 452–459. https://doi.org/10.1038/s41929-018-0082-9

Journal Article Type Article
Acceptance Date Apr 24, 2018
Online Publication Date Jun 4, 2018
Publication Date Jun 4, 2018
Deposit Date Jun 6, 2018
Publicly Available Date Mar 29, 2024
Journal Nature Catalysis
Electronic ISSN 2520-1158
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 1
Pages 452–459
DOI https://doi.org/10.1038/s41929-018-0082-9
Keywords Biocatalysis; Enzymes; Flow chemistry; Synthetic chemistry; methodology; Sustainability
Public URL https://nottingham-repository.worktribe.com/output/936457
Publisher URL https://www.nature.com/articles/s41929-018-0082-9

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