Martina L. Contente
Self-sustaining closed-loop multienzyme mediated conversion of amines into alcohols in continuous reactions
Contente, Martina L.; Paradisi, Francesca
Authors
Francesca Paradisi
Abstract
The synthesis of alcohols from amines starting material is an excellent, yet challenging, strategy for the preparation of pharmaceuticals and polymers. Here, we developed a versatile, self-sustaining closed-loop multienzymatic platform for the biocatalytic synthesis of a large range of non-commercially available products in continuous flow with excellent yields (80>99%), reaction times and optical purity of secondary alcohols (>99 e.e.). This process was also extended to the conversion of biogenic amines into high value alcohols, such as the powerful anti-oxidant hydroxytyrosol, and the synthesis of enantiopure 2-arylpropanols via dynamic kinetic resolution of commercially affordable racemic amines. The system exploits the in situ immobilisation of transaminases and redox enzymes which were combined to cater for a fully automated, ultra-efficient synthetic platform with cofactor recycling, in-line recovery of benign by-products and recirculation of the aqueous media containing the recycled cofactors in catalytic amount, which increases the efficiency of the system by over 20-fold.
Citation
Contente, M. L., & Paradisi, F. (2018). Self-sustaining closed-loop multienzyme mediated conversion of amines into alcohols in continuous reactions. Nature Catalysis, 1, 452–459. https://doi.org/10.1038/s41929-018-0082-9
Journal Article Type | Article |
---|---|
Acceptance Date | Apr 24, 2018 |
Online Publication Date | Jun 4, 2018 |
Publication Date | Jun 4, 2018 |
Deposit Date | Jun 6, 2018 |
Publicly Available Date | Dec 5, 2018 |
Journal | Nature Catalysis |
Electronic ISSN | 2520-1158 |
Publisher | Nature Publishing Group |
Peer Reviewed | Peer Reviewed |
Volume | 1 |
Pages | 452–459 |
DOI | https://doi.org/10.1038/s41929-018-0082-9 |
Keywords | Biocatalysis; Enzymes; Flow chemistry; Synthetic chemistry; methodology; Sustainability |
Public URL | https://nottingham-repository.worktribe.com/output/936457 |
Publisher URL | https://www.nature.com/articles/s41929-018-0082-9 |
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