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Isolation and characterization of novel broad host range bacteriophages of Vibrio cholerae O1 from Bengal

Sarkar, BanwarilalL; Sarkar, Sounak; Das, Mayukh; Bhowmick, TusharSuvra; Koley, Hemanta; Atterbury, Robert; Chakrabarti, AlokK

Authors

BanwarilalL Sarkar

Sounak Sarkar

Mayukh Das

TusharSuvra Bhowmick

Hemanta Koley

AlokK Chakrabarti



Abstract

Objectives: We have isolated a total of five newer cholera phages which are novel broad host range to incorporate with the existing phage typing schemes for an extended typing scheme. Materials and Methods: These newly isolated phages were well characterized including the electron micrograph. A total of 300 Vibrio cholerae strains were isolated from the different endemic region in India were included in phage typing study. Results: These phages were found different from the existing phages. Electron microscopic results showed that the phages belonged to myophage and podophage group. Characterization of the phages based on pH, temperature, and organic solvent sensitivity showed differences among the phages used in this study. All the strains of Vibrio O1 were typeable (100%) with the five set of cholera phages. Of these, 40% strains were clustered under Type-1. Conclusion: The newer Vibrio phages are novel and broad host range and will be useful to incorporate with the existing phage typing system for more precisely discriminate the strains of Vibrio cholerae.

Citation

Sarkar, B., Sarkar, S., Das, M., Bhowmick, T., Koley, H., Atterbury, R., & Chakrabarti, A. (2018). Isolation and characterization of novel broad host range bacteriophages of Vibrio cholerae O1 from Bengal. Journal of Global Infectious Diseases, 10(2), https://doi.org/10.4103/jgid.jgid_37_17

Journal Article Type Article
Acceptance Date Jul 25, 2017
Publication Date May 23, 2018
Deposit Date Jun 19, 2018
Publicly Available Date Jun 19, 2018
Journal Journal of Global Infectious Diseases
Print ISSN 0974-777X
Electronic ISSN 0974-8245
Publisher Medknow Publications
Peer Reviewed Peer Reviewed
Volume 10
Issue 2
DOI https://doi.org/10.4103/jgid.jgid_37_17
Keywords Bacteriophages, isolation, morphology, phage typing, plaque, Vibrio cholerae
Public URL http://eprints.nottingham.ac.uk/id/eprint/52497
Publisher URL http://dx.doi.org/10.4103/jgid.jgid_37_17
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nd-sa/4.0

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