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Rapid one-step biotinylation of biological and non-biological surfaces

Henry, Stephen; Williams, Eleanor; Barr, Katie; Korchagina, Elena; Tuzikov, Alexandr; Ilyushina, Natalia; Abayzeed, Sidahmed A.; Webb, Kevin F.; Bovin, Nicolai

Authors

Stephen Henry

Eleanor Williams

Katie Barr

Elena Korchagina

Alexandr Tuzikov

Natalia Ilyushina

Sidahmed A. Abayzeed

Kevin F. Webb kevin.webb@nottingham.ac.uk

Nicolai Bovin



Abstract

We describe a rapid one-step method to biotinylate virtually any biological or non-biological surface. Contacting a solution of biotin-spacer-lipid constructs with a surface will form a coating within seconds on non-biological surfaces or within minutes on most biological membranes including membrane viruses. The resultant biotinylated surface can then be used to interact with avidinylated conjugates, beads, vesicles, surfaces or cells.

Journal Article Type Article
Publication Date Feb 12, 2018
Journal Scientific Reports
Print ISSN 2045-2322
Electronic ISSN 2045-2322
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 8
Issue 1
Article Number 2845
APA6 Citation Henry, S., Williams, E., Barr, K., Korchagina, E., Tuzikov, A., Ilyushina, N., …Bovin, N. (2018). Rapid one-step biotinylation of biological and non-biological surfaces. Scientific Reports, 8(1), https://doi.org/10.1038/s41598-018-21186-3
DOI https://doi.org/10.1038/s41598-018-21186-3
Publisher URL https://doi.org/10.1038/s41598-018-21186-3
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Scientific Reports Biotinilysation.pdf (1.1 Mb)
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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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