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Role of geometrical cues in bone marrow-derived mesenchymal stem cell survival, growth and osteogenic differentiation

Gupta, Dhanak; Grant, David M.; Hossain, Kazi M. Zakir; Ahmed, Ifty; Sottile, Virginie

Authors

Dhanak Gupta

DAVID GRANT DAVID.GRANT@NOTTINGHAM.AC.UK
Professor of Materials Science

Kazi M. Zakir Hossain

Virginie Sottile



Abstract

Mesenchymal stem cells play a vital role in bone formation process by differentiating into osteoblasts, in a tissue that offers not a flat but a discontinuous three-dimensional (3D) topography in vivo. In order to understand how geometry may be affecting mesenchymal stem cells, this study explored the influence of 3D geometry on mesenchymal stem cell-fate by comparing cell growth, viability and osteogenic potential using monolayer (two-dimensional, 2D) with microsphere (3D) culture systems normalised to surface area. The results suggested lower cell viability and reduced cell growth in 3D. Alkaline phosphatase activity was higher in 3D; however, both collagen and mineral deposition appeared significantly lower in 3D, even after osteogenic supplementation. Also, there were signs of patchy mineralisation in 3D with or without osteogenic supplementation as early as day 7. These results suggest that the convex surfaces on microspheres and inter-particulate porosity may have led to variable cell morphology and fate within the 3D culture. This study provides deeper insights into geometrical regulation of mesenchymal stem cell responses applicable for bone tissue engineering.

Citation

Gupta, D., Grant, D. M., Hossain, K. M. Z., Ahmed, I., & Sottile, V. (2018). Role of geometrical cues in bone marrow-derived mesenchymal stem cell survival, growth and osteogenic differentiation. Journal of Biomaterials Applications, 32(7), https://doi.org/10.1177/0885328217745699

Journal Article Type Article
Acceptance Date Nov 10, 2017
Online Publication Date Dec 13, 2017
Publication Date Feb 1, 2018
Deposit Date Jan 11, 2018
Publicly Available Date Mar 29, 2024
Journal Journal of Biomaterials Applications
Print ISSN 0885-3282
Electronic ISSN 1530-8022
Publisher SAGE Publications
Peer Reviewed Peer Reviewed
Volume 32
Issue 7
DOI https://doi.org/10.1177/0885328217745699
Keywords Mesenchymal stem cells; microspheres; substrate geometry; osteogenesis; 3D culture
Public URL https://nottingham-repository.worktribe.com/output/909123
Publisher URL http://journals.sagepub.com/doi/10.1177/0885328217745699

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