Skip to main content

Research Repository

Advanced Search

Evaluating fibre orientation dispersion in white matter: comparison of diffusion MRI, histology and polarized light imaging

Mollink, Jeroen; Kleinnijenhuis, Michiel; Cappellen van Walsum, Anne-Marie van; Sotiropoulos, Stamatios N.; Cottaar, Michiel; Mirfin, Christopher; Heinrich, Mattias P.; Jenkinson, Mark; Pallebage-Gamarallage, Menuka; Ansorge, Olaf; Jbabdi, Saad; Miller, Karla L.

Authors

Jeroen Mollink

Michiel Kleinnijenhuis

Anne-Marie van Cappellen van Walsum

Stamatios N. Sotiropoulos

Michiel Cottaar

Christopher Mirfin

Mattias P. Heinrich

Mark Jenkinson

Menuka Pallebage-Gamarallage

Olaf Ansorge

Saad Jbabdi

Karla L. Miller



Abstract

Diffusion MRI is an exquisitely sensitive probe of tissue microstructure, and is currently the only non-invasive measure of the brain’s fibre architecture. As this technique becomes more sophisticated and microstructurally informative, there is increasing value in comparing diffusion MRI with microscopic imaging in the same tissue samples. This study compared estimates of fibre orientation dispersion in white matter derived from diffusion MRI to reference measures of dispersion obtained from polarized light imaging and histology.

Three post-mortem brain specimens were scanned with diffusion MRI and analyzed with a two-compartment dispersion model. The specimens were then sectioned for microscopy, including polarized light imaging estimates of fibre orientation and histological quantitative estimates of myelin and astrocytes. Dispersion estimates were correlated on region – and voxel-wise levels in the corpus callosum, the centrum semiovale and the corticospinal tract.

The region-wise analysis yielded correlation coefficients of r=0.79 for the diffusion MRI and histology comparison, while r=0.60 was reported for the comparison with polarized light imaging. In the corpus callosum, we observed a pattern of higher dispersion at the midline compared to its lateral aspects. This pattern was present in all modalities and the dispersion profiles from microscopy and diffusion MRI were highly correlated. The astrocytes appeared to have minor contribution to dispersion observed with diffusion MRI.

These results demonstrate that fibre orientation dispersion estimates from diffusion MRI represents the tissue architecture well. Dispersion models might be improved by more faithfully incorporating an informed mapping based on microscopy data.

Journal Article Type Article
Publication Date Aug 15, 2017
Journal NeuroImage
Print ISSN 1053-8119
Electronic ISSN 1095-9572
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 157
APA6 Citation Mollink, J., Kleinnijenhuis, M., Cappellen van Walsum, A. V., Sotiropoulos, S. N., Cottaar, M., Mirfin, C., …Miller, K. L. (2017). Evaluating fibre orientation dispersion in white matter: comparison of diffusion MRI, histology and polarized light imaging. NeuroImage, 157, https://doi.org/10.1016/j.neuroimage.2017.06.001
DOI https://doi.org/10.1016/j.neuroimage.2017.06.001
Keywords Dispersion; Diffusion MRI; Post-mortem; Polarized Light Imaging; Myelin; Astrocytes; Validation
Publisher URL http://www.sciencedirect.com/science/article/pii/S1053811917304706
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

Files

White matter 1-s2.0-S1053811917304706-main.pdf (4.6 Mb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0







You might also like



Downloadable Citations

;