Lucky Legbosi Nwidu
Anti-acetylcholinesterase activity and antioxidant properties of extracts and fractions of Carpolobia lutea
Nwidu, Lucky Legbosi; Elmorsy, Ekramy; Thornton, Jack; Wijamunige, Buddhika; Wijesekara, Anusha; Tarbox, Rebecca; Warren, Averil; Carter, Wayne
Authors
Ekramy Elmorsy
Jack Thornton
Buddhika Wijamunige
Anusha Wijesekara
Rebecca Tarbox
Averil Warren
WAYNE CARTER wayne.carter@nottingham.ac.uk
Associate Professor
Abstract
Context: There is an unmet need to discover new treatments for Alzheimer’s disease. This study determined the anti-acetylcholinesterase (AChE) activity, DPPH free radical scavenging and antioxidant properties of Carpolobia lutea G. Don (Polygalaceae).
Objective: To quantify C. lutea anti-AChE, DPPH free radical scavenging, and antioxidant activities, and cell cytotoxicity.
Materials and methods: Plant stem, leaves, and roots were subjected to sequential solvent extractions, and screened for anti-AChE activity across a concentration range of 0.02-200 μg//mL. Plant DPPH radical scavenging activity, reducing power, and total phenolic and flavonoid content were determined, and cytotoxicity evaluated using human hepatocytes.
Results: C. lutea exhibited concentration dependent anti-AChE activity. The most potent inhibitory activity for the stem was the crude ethanol extract and hexane stem fraction oil (IC₅₀ = 140 μg/mL); for the leaves the chloroform leaf fraction (IC₅₀ = 60 μg/mL/mL); and for roots, the methanol, ethyl acetate, and aqueous root fractions (IC₅₀ = 0.3-3 μg/mL). Dose-dependent free radical scavenging activity and reducing power were observed with increasing stem, leaf, or root concentration. Total phenolics were highest in the stem: ~632 mg gallic acid equivalents/g for a hexane stem fraction oil. Total flavonoid content was highest in the leaves: ~297 mg quercetin equivalents/g for a chloroform leaf fraction. At 1 μg/mL, only the crude ethanol extract oil was significantly cytotoxic to hepatocytes.
Discussion and conclusion: C. lutea possesses anti-AChE activity and beneficial antioxidant capacity indicative of its potential development as a treatment of Alzheimer’s and other diseases characterized by a cholinergic deficit.
Citation
Nwidu, L. L., Elmorsy, E., Thornton, J., Wijamunige, B., Wijesekara, A., Tarbox, R., …Carter, W. (2017). Anti-acetylcholinesterase activity and antioxidant properties of extracts and fractions of Carpolobia lutea. Pharmaceutical Biology, 55(1), https://doi.org/10.1080/13880209.2017.1339283
Journal Article Type | Article |
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Acceptance Date | Jun 3, 2017 |
Publication Date | Jun 19, 2017 |
Deposit Date | Jun 13, 2017 |
Publicly Available Date | Jun 19, 2017 |
Journal | Pharmaceutical Biology |
Print ISSN | 1388-0209 |
Electronic ISSN | 1744-5116 |
Publisher | Taylor & Francis Open |
Peer Reviewed | Peer Reviewed |
Volume | 55 |
Issue | 1 |
DOI | https://doi.org/10.1080/13880209.2017.1339283 |
Keywords | Anti-acetylcholinesterase; Carpolobia lutea; Antioxidant; Alzheimer's disease |
Public URL | https://nottingham-repository.worktribe.com/output/866899 |
Publisher URL | https://www.tandfonline.com/doi/full/10.1080/13880209.2017.1339283 |
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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0