Sheeba Irshad
RORγt+ innate lymphoid cells promote lymph node metastasis of breast cancers
Irshad, Sheeba; Flores-Borja, Fabian; Lawler, Katherine; Monypenny, James; Evans, Rachel; Male, Victoria; Gordon, Peter; Cheung, Anthony; Gazinska, Patrycja; Noor, Farzana; Wong, Felix; Grigoriadis, Anita; Fruhwirth, Gilbert O.; Barber, Paul R.; Woodman, Natalie; Martin, Stewart G.
Authors
Fabian Flores-Borja
Katherine Lawler
James Monypenny
Rachel Evans
Victoria Male
Peter Gordon
Anthony Cheung
Patrycja Gazinska
Farzana Noor
Felix Wong
Anita Grigoriadis
Gilbert O. Fruhwirth
Paul R. Barber
Natalie Woodman
STEWART MARTIN STEWART.MARTIN@NOTTINGHAM.AC.UK
Professor of Cancer and Radiation Biology
Abstract
Cancer cells tend to metastasize first to tumor-draining lymph nodes, but the mechanisms mediating cancer cell invasion into the lymphatic vasculature remain little understood. Here, we show that in the human breast tumor microenvironment (TME), the presence of increased numbers of RORγt+ group 3 innate lymphoid cells (ILC3) correlates with an increased likelihood of lymph node metastasis. In a preclinical mouse model of breast cancer, CCL21-mediated recruitment of ILC3 to tumors stimulated the production of the CXCL13 by TME stromal cells, which in turn promoted ILC3–stromal interactions and production of the cancer cell motile factor RANKL. Depleting ILC3 or neutralizing CCL21, CXCL13, or RANKL was sufficient to decrease lymph node metastasis. Our findings establish a role for RORγt+ILC3 in promoting lymphatic metastasis by modulating the local chemokine milieu of cancer cells in the TME.
Citation
Irshad, S., Flores-Borja, F., Lawler, K., Monypenny, J., Evans, R., Male, V., …Martin, S. G. (2017). RORγt+ innate lymphoid cells promote lymph node metastasis of breast cancers. Cancer Research, 77(5), 1083-1096. https://doi.org/10.1158/0008-5472.CAN-16-0598
Journal Article Type | Article |
---|---|
Acceptance Date | Dec 10, 2016 |
Online Publication Date | Jan 12, 2017 |
Publication Date | Mar 31, 2017 |
Deposit Date | May 24, 2017 |
Publicly Available Date | May 24, 2017 |
Journal | Cancer Research |
Print ISSN | 0008-5472 |
Electronic ISSN | 1538-7445 |
Publisher | American Association for Cancer Research |
Peer Reviewed | Peer Reviewed |
Volume | 77 |
Issue | 5 |
Pages | 1083-1096 |
DOI | https://doi.org/10.1158/0008-5472.CAN-16-0598 |
Public URL | https://nottingham-repository.worktribe.com/output/853163 |
Publisher URL | http://cancerres.aacrjournals.org/content/77/5/1083 |
Additional Information | 33 authors in total. |
Contract Date | May 24, 2017 |
Files
SI et al Revised_Manuscript_Final2.pdf
(317 Kb)
PDF
SI et al Supplementary_Figures_Tables_.pdf
(3.4 Mb)
PDF
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