Thioredoxin System Protein Expression in Carcinomas of the Pancreas, Distal Bile Duct, and Ampulla in the United Kingdom

Al-Hadyan, Khaled S.; Storr, Sarah J.; Zaitoun, Abed M.; Lobo, Dileep N.; Martin, Stewart G.

Abstract

Background: Poor survival outcomes in periampullary cancer highlight the need for improvement in biomarkers and the development of novel therapies. Redox proteins, including the thioredoxin system, play vital roles in cellular antioxidant systems. Methods: In this retrospective study, thioredoxin (Trx), thioredoxin-interacting protein (TxNIP), and thioredoxin reductase (TrxR) protein expression was assessed in 85 patients with pancreatic ductal adenocarcinoma (PDAC) and 145 patients with distal bile duct or ampullary carcinoma using conventional immunohistochemistry. Results: In patients with PDAC, high cytoplasmic TrxR expression was significantly associated with lymph node metastasis (p = 0.033). High cytoplasmic and nuclear Trx expression was significantly associated with better overall survival (p = 0.018 and p = 0.006, respectively), and nuclear Trx expression remained significant in multivariate Cox regression analysis (p < 0.0001). In distal bile duct and ampullary carcinomas, high nuclear TrxR expression was associated with vascular (p = 0.001) and perineural (p = 0.021) invasion, and low cytoplasmic TxNIP expression was associated with perineural invasion (p = 0.025). High cytoplasmic TxNIP expression was significantly associated with better overall survival (p = 0.0002), which remained significant in multivariate Cox regression analysis (p = 0.013). Conclusions: These findings demonstrate the prognostic importance of Trx system protein expression in periampullary cancers.

Citation

Al-Hadyan, K. S., Storr, S. J., Zaitoun, A. M., Lobo, D. N., & Martin, S. G. (2024). Thioredoxin System Protein Expression in Carcinomas of the Pancreas, Distal Bile Duct, and Ampulla in the United Kingdom. Diseases, 12(10), Article 227. https://doi.org/10.3390/diseases12100227

• Journal Article Type: Article
• Acceptance Date: Sep 21, 2024
• Online Publication Date: Sep 24, 2024
• Publication Date: 2024-10
• Deposit Date: Sep 23, 2024
• Publicly Available Date: Sep 24, 2024
• Journal: Diseases
• Electronic ISSN: 2079-9721
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 12
• Issue: 10
• Article Number: 227
• DOI: https://doi.org/10.3390/diseases12100227
• Keywords: thioredoxin; redox protein; periampullary cancer; pancreatic ductal adenocarcinoma; ampullary cancer; bile duct cancer
• Public URL: https://nottingham-repository.worktribe.com/output/39983136
• Publisher URL: https://www.mdpi.com/2079-9721/12/10/227