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Refinement of the taxonomic structure of 16SrXI and 16SrXIV phytoplasmas of gramineous plants using multilocus sequence typing

Abeysinghe, Saman; Abeysinghe, Pushpa Damayanthi; Kanatiwela-de Silva, Chamini; Udagama, Preethi; Warawichanee, Kanjana; Aljafar, Naofel; Kawicha, Praphat; Dickinson, Matthew

Authors

Saman Abeysinghe

Pushpa Damayanthi Abeysinghe

Chamini Kanatiwela-de Silva

Preethi Udagama

Kanjana Warawichanee

Naofel Aljafar

Praphat Kawicha



Abstract

Phytoplasmas that infect gramineous plants, including Napier grass stunt, sugarcane whiteleaf, sugarcane grassy shoot, and Bermuda grass whiteleaf, have been classified into two closely related groups, 16SrXI and 16SrXIV, based on the 16S ribosomal RNA (rRNA) gene. Subsequently, phytoplasmas associated with coconut and Areca palm in southern India and Sri Lanka have been added into the 16SrXI group. However, the 16S rRNA gene gives relatively poor resolution between these phytoplasmas. In this study, a new set of universal phytoplasma primers that amplify approximately 1 kb of the leucyl transfer RNA synthetase (leuS) gene have been validated on a broad range of phytoplasma taxonomic groups. These have been used along with partial sequences of the secA gene to clarify the taxonomic classification of 16SrXI and 16SrXIV phytoplasmas. Based on this data, the sugarcane whiteleaf and grassy shoot phytoplasmas appear to be the same phytoplasma. The Napier grass stunt phytoplasma forms a distinct group from the Bermuda grass whiteleaf and sugarcane phytoplasmas, suggesting that Napier grass stunt should be in its own ‘Candidatus Phytoplasma sp.’. The phytoplasmas associated with coconut and arecanut in southern India and Sri Lanka, which are in the same 16SrXI group, appear in different groups based on secA analysis.

Journal Article Type Article
Publication Date Oct 31, 2016
Journal Plant Disease
Print ISSN 0191-2917
Electronic ISSN 0191-2917
Publisher American Phytopathological Society
Peer Reviewed Not Peer Reviewed
Volume 100
Issue 10
APA6 Citation Abeysinghe, S., Abeysinghe, P. D., Kanatiwela-de Silva, C., Udagama, P., Warawichanee, K., Aljafar, N., …Dickinson, M. (2016). Refinement of the taxonomic structure of 16SrXI and 16SrXIV phytoplasmas of gramineous plants using multilocus sequence typing. Plant Disease, 100(10), doi:10.1094/PDIS-02-16-0244-RE
DOI https://doi.org/10.1094/PDIS-02-16-0244-RE
Publisher URL http://apsjournals.apsnet.org/doi/10.1094/PDIS-02-16-0244-RE
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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