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A paper-based in vitro model for on-chip investigation of the human respiratory system

Rahimi, Rahim; Htwe, Su Su; Ochoa, Manuel; Donaldson, Amy; Zieger, Michael; Sood, Rajiv; Tamayol, Ali; Khademhosseini, Ali; Ghaemmaghami, Amir M.; Ziaie, Babak

Authors

Rahim Rahimi

Su Su Htwe mrxsshtw1@nottingham.ac.uk

Manuel Ochoa

Amy Donaldson

Michael Zieger

Rajiv Sood

Ali Tamayol

Ali Khademhosseini

Babak Ziaie



Abstract

Culturing cells at the air–liquid interface (ALI) is essential for creating functional in vitro models of lung tissues. We present the use of direct-patterned laser-treated hydrophobic paper as an effective semi-permeable membrane, ideal for ALI cell culture. The surface properties of the paper are modified through a selective CO2 laser-assisted treatment to create a unique porous substrate with hydrophilic regions that regulate fluid diffusion and cell attachment. To select the appropriate model, four promising hydrophobic films were compared with each other in terms of gas permeability and long-term strength in an aqueous environment (wet-strength). Among the investigated substrates, parchment paper showed the fastest rate of oxygen permeability (3 times more than conventional transwell cell culture membranes), with the least variation in its dry and wet tensile strengths (124 MPa and 58 MPa, remaining unchanged after 7 days of submersion in PBS).The final paper-based platform provides an ideal, robust, and inexpensive device for generating monolayers of lung epithelial cells on-chip in a high-throughput fashion for disease modelling and in vitro drug testing.

Citation

Rahimi, R., Htwe, S. S., Ochoa, M., Donaldson, A., Zieger, M., Sood, R., …Ziaie, B. (in press). A paper-based in vitro model for on-chip investigation of the human respiratory system. Lab on a Chip, 16(22), https://doi.org/10.1039/C6LC00866F

Journal Article Type Article
Acceptance Date Sep 26, 2016
Online Publication Date Sep 27, 2016
Deposit Date Oct 18, 2016
Publicly Available Date Oct 18, 2016
Journal Lab on a Chip
Print ISSN 1473-0197
Electronic ISSN 1473-0189
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 16
Issue 22
DOI https://doi.org/10.1039/C6LC00866F
Public URL http://eprints.nottingham.ac.uk/id/eprint/37637
Publisher URL http://dx.doi.org/10.1039/C6LC00866F
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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