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Novel highly sensitive protein sensors based on tapered optical fibres modified with Au-based nanocoatings

Urrutia, Aitor; Bojan, Kartheka; Marques, Leonel; Mullaney, Kevin; Goicoechea, Javier; James, Stephen; Clark, Matt; Korposh, Sergiy; Tatam, Ralph

Authors

Aitor Urrutia

Kartheka Bojan

Leonel Marques

Kevin Mullaney

Javier Goicoechea

Stephen James

MATT CLARK matt.clark@nottingham.ac.uk
Professor of Applied Optics

Ralph Tatam



Abstract

Novel protein sensors based on tapered optical fibres modified with Au coatings deposited using two different procedures are proposed. Au-based coatings are deposited onto a nonadiabatic tapered optical fibre using (i) a novel facile method composed of layer-by-layer deposition consisting of polycation (poly(allylamine hydrochloride), PAH) and negatively charged SiO₂ nanoparticles (NPs) followed by the deposition of the charged Au NPs and (ii) the sputtering technique.The Au NPs and Au thin film surfaces are then modified with biotin in order to bind streptavidin (SV) molecules and detect them. The sensing principle is based on the sensitivity of the transmission spectrum of the device to changes in the refractive index of the coatings induced by the SV binding to the biotin. Both sensors showed high sensitivity to SV, with the lowest measured concentration levels below 2.5 nM. The calculated binding constant for the biotin-SV pair was 2.2×10‾¹¹ M‾¹ when a tapered fibre modified with the LbL method was used, with a limit of detection (LoD) of 271 pM. The sensor formed using sputtering had a binding constant of 1.01 × 10‾¹⁰ M‾¹ with a LoD of 806 pM. These new structures and their simple fabrication technique could be used to develop other biosensors.

Citation

Urrutia, A., Bojan, K., Marques, L., Mullaney, K., Goicoechea, J., James, S., …Tatam, R. (in press). Novel highly sensitive protein sensors based on tapered optical fibres modified with Au-based nanocoatings. Journal of Sensors, 2016, https://doi.org/10.1155/2016/8129387

Journal Article Type Article
Acceptance Date Jul 19, 2016
Online Publication Date Aug 18, 2016
Deposit Date Nov 28, 2016
Publicly Available Date Nov 28, 2016
Journal Journal of Sensors
Print ISSN 1687-725X
Electronic ISSN 1687-7268
Publisher Hindawi Publishing Corporation
Peer Reviewed Peer Reviewed
Volume 2016
DOI https://doi.org/10.1155/2016/8129387
Public URL http://eprints.nottingham.ac.uk/id/eprint/38986
Publisher URL https://www.hindawi.com/journals/js/2016/8129387/
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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