Immunomodulation with self-crosslinked polyelectrolyte multilayer-based coatings

Knopf-Marques, Helena; Singh, Sonali; Htwe, Su Su; Wolfova, Lucie; Buffa, Radovan; Bacharouche, Jalal; Francius, Gre?gory; Voegel, Jean-Claude; Schaaf, Pierre; Ghaemmaghami, Amir M.; Vrana, Nihal E.; Lavalle, Philippe

Abstract

This study aims to design an optimal polyelectrolyte multilayer film of poly-L-lysine (PLL) and hyaluronic acid(HA) as an anti-inflammatory cytokine release system in order to decrease the implant failure due to any immune reactions. The chemical modification of the HA with aldehyde moieties allows self-cross-linking of the film and an improvement in the mechanical properties of the film. The cross-linking of the film and the release of immunomodulatory cytokine (IL-4) stimulate the differentiation of primary human monocytes seeded on the films into pro-healing macrophages phenotype. This induces the production of antiinflammatory cytokines (IL1-RA and CCL18) and the decrease of proinflammatory cytokines secreted (IL-12, TNF-α, and IL-1β). Moreover, we demonstrate that cross-linking PLL/HA film using HA-aldehyde is already effective by itself to limit inflammatory processes. Finally, this functionalized self-cross-linked PLL/HA-aldehyde films constitutes an innovative and efficient candidate for immunomodulation of any kind of implants of various architecture and properties.

Citation

Knopf-Marques, H., Singh, S., Htwe, S. S., Wolfova, L., Buffa, R., Bacharouche, J., …Lavalle, P. (2016). Immunomodulation with self-crosslinked polyelectrolyte multilayer-based coatings. Biomacromolecules, 17(6), 2189-2198. https://doi.org/10.1021/acs.biomac.6b00429

• Journal Article Type: Article
• Acceptance Date: May 16, 2016
• Online Publication Date: May 16, 2016
• Publication Date: Jun 13, 2016
• Deposit Date: Jul 7, 2016
• Publicly Available Date: Jul 7, 2016
• Journal: Biomacromolecules
• Print ISSN: 1525-7797
• Electronic ISSN: 1525-7797
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 17
• Issue: 6
• Pages: 2189-2198
• DOI: https://doi.org/10.1021/acs.biomac.6b00429
• Public URL: https://nottingham-repository.worktribe.com/output/795360
• Publisher URL: http://pubs.acs.org/doi/abs/10.1021/acs.biomac.6b00429
• Additional Information: This document is the Accepted Manuscript version of a Published Work that appeared in final form in Biomacromolecules, copyright ©2016 American Chemical Society after peer review and technical editing by the publisher.