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Immunomodulation with self-crosslinked polyelectrolyte multilayer-based coatings

Knopf-Marques, Helena; Singh, Sonali; Htwe, Su Su; Wolfova, Lucie; Buffa, Radovan; Bacharouche, Jalal; Francius, Grégory; Voegel, Jean-Claude; Schaaf, Pierre; Ghaemmaghami, Amir M.; Vrana, Nihal E.; Lavalle, Philippe

Authors

Helena Knopf-Marques helenamarque@gmail.com

Su Su Htwe mrxsshtw1@nottingham.ac.uk

Lucie Wolfova Lucie.wolfova@contipro.com

Radovan Buffa radovan.buffa@contipro.com

Jalal Bacharouche jalal.bacharouche@univ-lorraine.fr

Grégory Francius gregory.francius@univ-lorraine.fr

Jean-Claude Voegel jean-claude.voegel@inserm.fr

Pierre Schaaf pierre.schaaf@inserm.fr

Nihal E. Vrana e.vrana@protipmedical.com

Philippe Lavalle philippe.lavalle@inserm.fr



Abstract

This study aims to design an optimal polyelectrolyte multilayer film of poly-L-lysine (PLL) and hyaluronic acid(HA) as an anti-inflammatory cytokine release system in order to decrease the implant failure due to any immune reactions. The chemical modification of the HA with aldehyde moieties allows self-cross-linking of the film and an improvement in the mechanical properties of the film. The cross-linking of the film and the release of immunomodulatory cytokine (IL-4) stimulate the differentiation of primary human monocytes seeded on the films into pro-healing macrophages phenotype. This induces the production of antiinflammatory cytokines (IL1-RA and CCL18) and the decrease of proinflammatory cytokines secreted (IL-12, TNF-α, and IL-1β). Moreover, we demonstrate that cross-linking PLL/HA film using HA-aldehyde is already effective by itself to limit inflammatory processes. Finally, this functionalized self-cross-linked PLL/HA-aldehyde films constitutes an innovative and efficient candidate for immunomodulation of any kind of implants of various architecture and properties.

Citation

Knopf-Marques, H., Singh, S., Htwe, S. S., Wolfova, L., Buffa, R., Bacharouche, J., …Lavalle, P. (2016). Immunomodulation with self-crosslinked polyelectrolyte multilayer-based coatings. Biomacromolecules, 17(6), https://doi.org/10.1021/acs.biomac.6b00429

Journal Article Type Article
Acceptance Date May 16, 2016
Online Publication Date May 16, 2016
Publication Date Jun 13, 2016
Deposit Date Jul 7, 2016
Publicly Available Date Jul 7, 2016
Journal Biomacromolecules
Print ISSN 1525-7797
Electronic ISSN 1525-7797
Publisher American Chemical Society
Peer Reviewed Peer Reviewed
Volume 17
Issue 6
DOI https://doi.org/10.1021/acs.biomac.6b00429
Public URL http://eprints.nottingham.ac.uk/id/eprint/34683
Publisher URL http://pubs.acs.org/doi/abs/10.1021/acs.biomac.6b00429
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc/4.0
Additional Information This document is the Accepted Manuscript version of a Published Work that appeared in final form in Biomacromolecules, copyright ©2016 American Chemical Society after peer review and technical editing by the publisher.

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc/4.0





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