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The mannose receptor negatively modulates the Toll-like receptor 4–aryl hydrocarbon receptor–indoleamine 2,3-dioxygenase axis in dendritic cells affecting T helper cell polarization

Salazar, Fabi�n; Hall, Laurence; Negm, Ola H.; Awuah, Dennis; Tighe, Patrick J.; Shakib, Farouk; Ghaemmaghami, Amir M.

The mannose receptor negatively modulates the Toll-like receptor 4–aryl hydrocarbon receptor–indoleamine 2,3-dioxygenase axis in dendritic cells affecting T helper cell polarization Thumbnail


Authors

Fabi�n Salazar

Laurence Hall

Ola H. Negm

Dennis Awuah

PATRICK TIGHE paddy.tighe@nottingham.ac.uk
Professor of Molecular Immunology

Farouk Shakib



Abstract

Background: Dendritic cells (DCs) are key players in the induction and re-elicitation of TH2 responses to allergens. We have previously shown that different C-type lectin receptors on DCs play a major role in allergen recognition and uptake. In particular, mannose receptor (MR), through modulation of Toll-like receptor (TLR) 4 signaling, can regulate indoleamine 2,3-dioxygenase (IDO) activity, favoring TH2 responses. Interestingly, the aryl hydrocarbon receptor (AhR), a ligand-dependent transcription factor with an emerging role in immune modulation, has been implicated in IDO activation in response to TLR stimulation.
Objective: Here we investigated how allergens and lectins modulate the TLR4-AhR-IDO axis in human monocyte-derived DCs.
Methods: Using a combination of genomics, proteomics, and immunologic studies, we investigated the role of MR and AhR in IDO regulation and its effect on T helper cell differentiation.
Results: We have demonstrated that LPS induces both IDO isoforms (IDO1 and IDO2) in DCs, with partial involvement of AhR. Additionally, we found that, like mannan, different airborne allergens can effectively downregulate TLR4-induced IDO1 and IDO2 expression, most likely through binding to the MR. Mannose-based ligands were also able to downregulate IL-12p70 production by DCs, affecting T helper cell polarization. Interestingly, AhR and some components of the noncanonical nuclear factor ?B pathway were shown to be downregulated after MR engagement, which could explain the regulatory effects of MR on IDO expression.
Conclusion: Our work demonstrates a key role for MR in the modulation of the TLR4-AhR-IDO axis, which has a significant effect on DC behavior and the development of immune responses against allergens.

Journal Article Type Article
Acceptance Date Oct 27, 2015
Online Publication Date Dec 17, 2015
Publication Date 2016-06
Deposit Date Aug 3, 2016
Publicly Available Date Aug 3, 2016
Journal Journal of Allergy and Clinical Immunology
Print ISSN 0091-6749
Electronic ISSN 1097-6825
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 137
Issue 6
Pages 1841-1851.e2
DOI https://doi.org/10.1016/j.jaci.2015.10.033
Keywords Dendritic cells; allergy; TH2; indoleamine 2,3-dioxygenase; C-type lectin receptor; mannose receptor; Toll-like receptor 4; aryl-hydrocarbon receptor; nuclear factor ?B
Public URL https://nottingham-repository.worktribe.com/output/787395
Publisher URL http://www.sciencedirect.com/science/article/pii/S0091674915016425

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