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Genome-wide profiling in treatment-naive early rheumatoid arthritis reveals DNA methylome changes in T and B lymphocytes

Glossop, John R.; Emes, Richard D.; Nixon, Nicola B.; Packham, Jon C.; Fryer, Anthony A.; Mattey, Derek L.; Farrell, William E.

Authors

John R. Glossop

Richard D. Emes

Nicola B. Nixon

Jon C. Packham

Anthony A. Fryer

Derek L. Mattey

William E. Farrell



Abstract

AIM: Although aberrant DNA methylation has been described in rheumatoid arthritis (RA), no studies have interrogated this epigenetic modification in early disease. Following recent investigations of T- and B-lymphocytes in established disease, we now characterize in these cell populations genome-wide DNA methylation in treatment-naive patients with early RA.
PATIENTS & METHODS: HumanMethylation450 BeadChips were used to examine genome-wide DNA methylation in lymphocyte populations from 23 early RA patients and 11 healthy individuals.
RESULTS: Approximately 2000 CpGs in each cell type were differentially methylated in early RA. Clustering analysis identified a novel methylation signature in each cell type (150 sites in T-lymphocytes, 113 sites in B-lymphocytes) that clustered all patients separately from controls. A subset of sites differentially methylated in early RA displayed similar changes in established disease.
CONCLUSION: Treatment-naive early RA patients display novel disease-specific DNA methylation aberrations, supporting a potential role for these changes in the development of RA.

Journal Article Type Article
Publication Date Feb 1, 2016
Journal Epigenomics
Print ISSN 1750-1911
Electronic ISSN 1750-192X
Publisher Future Medicine
Peer Reviewed Peer Reviewed
Volume 8
Issue 2
APA6 Citation Glossop, J. R., Emes, R. D., Nixon, N. B., Packham, J. C., Fryer, A. A., Mattey, D. L., & Farrell, W. E. (2016). Genome-wide profiling in treatment-naive early rheumatoid arthritis reveals DNA methylome changes in T and B lymphocytes. Epigenomics, 8(2), https://doi.org/10.2217/epi.15.103
DOI https://doi.org/10.2217/epi.15.103
Keywords Early rheumatoid arthritis; Treatment-naïve; DNA methylation; T-lymphocyte; B-lymphocyte; CpG; Genome-wide; Illumina 450K array.
Publisher URL http://www.futuremedicine.com/doi/10.2217/epi.15.103
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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