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Genome-wide profiling in treatment-naive early rheumatoid arthritis reveals DNA methylome changes in T and B lymphocytes

Glossop, John R.; Emes, Richard D.; Nixon, Nicola B.; Packham, Jon C.; Fryer, Anthony A.; Mattey, Derek L.; Farrell, William E.

Genome-wide profiling in treatment-naive early rheumatoid arthritis reveals DNA methylome changes in T and B lymphocytes Thumbnail


Authors

John R. Glossop

Richard D. Emes

Nicola B. Nixon

Jon C. Packham

Anthony A. Fryer

Derek L. Mattey

William E. Farrell



Abstract

AIM: Although aberrant DNA methylation has been described in rheumatoid arthritis (RA), no studies have interrogated this epigenetic modification in early disease. Following recent investigations of T- and B-lymphocytes in established disease, we now characterize in these cell populations genome-wide DNA methylation in treatment-naive patients with early RA.
PATIENTS & METHODS: HumanMethylation450 BeadChips were used to examine genome-wide DNA methylation in lymphocyte populations from 23 early RA patients and 11 healthy individuals.
RESULTS: Approximately 2000 CpGs in each cell type were differentially methylated in early RA. Clustering analysis identified a novel methylation signature in each cell type (150 sites in T-lymphocytes, 113 sites in B-lymphocytes) that clustered all patients separately from controls. A subset of sites differentially methylated in early RA displayed similar changes in established disease.
CONCLUSION: Treatment-naive early RA patients display novel disease-specific DNA methylation aberrations, supporting a potential role for these changes in the development of RA.

Citation

Glossop, J. R., Emes, R. D., Nixon, N. B., Packham, J. C., Fryer, A. A., Mattey, D. L., & Farrell, W. E. (2016). Genome-wide profiling in treatment-naive early rheumatoid arthritis reveals DNA methylome changes in T and B lymphocytes. Epigenomics, 8(2), https://doi.org/10.2217/epi.15.103

Journal Article Type Article
Online Publication Date Nov 10, 2015
Publication Date Feb 1, 2016
Deposit Date Dec 18, 2015
Publicly Available Date Dec 18, 2015
Journal Epigenomics
Print ISSN 1750-1911
Electronic ISSN 1750-192X
Publisher Future Medicine
Peer Reviewed Peer Reviewed
Volume 8
Issue 2
DOI https://doi.org/10.2217/epi.15.103
Keywords Early rheumatoid arthritis; Treatment-naïve; DNA methylation; T-lymphocyte; B-lymphocyte; CpG; Genome-wide; Illumina 450K array.
Public URL https://nottingham-repository.worktribe.com/output/770736
Publisher URL http://www.futuremedicine.com/doi/10.2217/epi.15.103
Contract Date Dec 18, 2015

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