John R. Glossop
Genome-wide profiling in treatment-naive early rheumatoid arthritis reveals DNA methylome changes in T and B lymphocytes
Glossop, John R.; Emes, Richard D.; Nixon, Nicola B.; Packham, Jon C.; Fryer, Anthony A.; Mattey, Derek L.; Farrell, William E.
Authors
Richard D. Emes
Nicola B. Nixon
Jon C. Packham
Anthony A. Fryer
Derek L. Mattey
William E. Farrell
Abstract
AIM: Although aberrant DNA methylation has been described in rheumatoid arthritis (RA), no studies have interrogated this epigenetic modification in early disease. Following recent investigations of T- and B-lymphocytes in established disease, we now characterize in these cell populations genome-wide DNA methylation in treatment-naive patients with early RA.
PATIENTS & METHODS: HumanMethylation450 BeadChips were used to examine genome-wide DNA methylation in lymphocyte populations from 23 early RA patients and 11 healthy individuals.
RESULTS: Approximately 2000 CpGs in each cell type were differentially methylated in early RA. Clustering analysis identified a novel methylation signature in each cell type (150 sites in T-lymphocytes, 113 sites in B-lymphocytes) that clustered all patients separately from controls. A subset of sites differentially methylated in early RA displayed similar changes in established disease.
CONCLUSION: Treatment-naive early RA patients display novel disease-specific DNA methylation aberrations, supporting a potential role for these changes in the development of RA.
Citation
Glossop, J. R., Emes, R. D., Nixon, N. B., Packham, J. C., Fryer, A. A., Mattey, D. L., & Farrell, W. E. (2016). Genome-wide profiling in treatment-naive early rheumatoid arthritis reveals DNA methylome changes in T and B lymphocytes. Epigenomics, 8(2), https://doi.org/10.2217/epi.15.103
Journal Article Type | Article |
---|---|
Online Publication Date | Nov 10, 2015 |
Publication Date | Feb 1, 2016 |
Deposit Date | Dec 18, 2015 |
Publicly Available Date | Mar 28, 2024 |
Journal | Epigenomics |
Print ISSN | 1750-1911 |
Electronic ISSN | 1750-192X |
Publisher | Future Medicine |
Peer Reviewed | Peer Reviewed |
Volume | 8 |
Issue | 2 |
DOI | https://doi.org/10.2217/epi.15.103 |
Keywords | Early rheumatoid arthritis; Treatment-naïve; DNA methylation; T-lymphocyte; B-lymphocyte; CpG; Genome-wide; Illumina 450K array. |
Public URL | https://nottingham-repository.worktribe.com/output/770736 |
Publisher URL | http://www.futuremedicine.com/doi/10.2217/epi.15.103 |
Files
Epigenomics 2015-2.pdf
(274 Kb)
PDF
You might also like
Genome Reference Assembly for Bottlenecked Southern Australian Koalas
(2022)
Journal Article
Downloadable Citations
About Repository@Nottingham
Administrator e-mail: digital-library-support@nottingham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search