Phenotyping pipeline reveals major seedling root growth QTL in hexaploid wheat

Atkinson, Jonathan A.; Wingen, Luzie U.; Griffiths, Marcus; Pound, Michael P.; Gaju, Oorbessy; Foulkes, M. John; Le Gouis, Jacques; Griffiths, Simon; Bennett, Malcolm J.; King, Julie; Wells, Darren M.

doi:10.1093/jxb/erv006

Authors

JONATHAN ATKINSON JONATHAN.ATKINSON@NOTTINGHAM.AC.UK
Future Food Beacon: technologist in Phenomics

Luzie U. Wingen

Marcus Griffiths

MICHAEL POUND Michael.Pound@nottingham.ac.uk
Associate Professor

Oorbessy Gaju

JOHN FOULKES john.foulkes@nottingham.ac.uk
Associate Professor

Jacques Le Gouis

Simon Griffiths

MALCOLM BENNETT malcolm.bennett@nottingham.ac.uk
Professor of Plant Science

Prof JULIE KING julie.king@nottingham.ac.uk
Professor of Cereal Genetics

DARREN WELLS DARREN.WELLS@NOTTINGHAM.AC.UK
Principal Research Fellow

Abstract

Seedling root traits of wheat (Triticum aestivum L.) have been shown to be important for efficient establishment and linked to mature plant traits such as height and yield. A root phenotyping pipeline, consisting of a germination paper-based screen combined with image segmentation and analysis software, was developed and used to characterize seedling traits in 94 doubled haploid progeny derived from a cross between the winter wheat cultivars Rialto and Savannah. Field experiments were conducted to measure mature plant height, grain yield, and nitrogen (N) uptake in three sites over 2 years. In total, 29 quantitative trait loci (QTLs) for seedling root traits were identified. Two QTLs for grain yield and N uptake co-localize with root QTLs on chromosomes 2B and 7D, respectively. Of the 29 root QTLs identified, 11 were found to co-localize on 6D, with four of these achieving highly significant logarithm of odds scores (>20). These results suggest the presence of a major-effect gene regulating seedling root vigour/growth on chromosome 6D.

Citation

Atkinson, J. A., Wingen, L. U., Griffiths, M., Pound, M. P., Gaju, O., Foulkes, M. J., …Wells, D. M. (2015). Phenotyping pipeline reveals major seedling root growth QTL in hexaploid wheat. Journal of Experimental Botany, 66(8), 2283-2292. https://doi.org/10.1093/jxb/erv006

Journal Article Type	Article
Acceptance Date	Dec 20, 2014
Online Publication Date	Mar 4, 2015
Publication Date	Jan 1, 2015
Deposit Date	Mar 29, 2017
Publicly Available Date	Mar 29, 2017
Journal	Journal of Experimental Botany
Print ISSN	0022-0957
Electronic ISSN	1460-2431
Publisher	Oxford University Press
Peer Reviewed	Peer Reviewed
Volume	66
Issue	8
Pages	2283-2292
DOI	https://doi.org/10.1093/jxb/erv006
Keywords	high-throughput phenotyping, root system architecture
Public URL	https://nottingham-repository.worktribe.com/output/748107
Publisher URL	https://academic.oup.com/jxb/article-lookup/doi/10.1093/jxb/erv006