An evolutionarily-unique heterodimeric voltage-gated cation channel found in aphids

Amey, Joanna S.; O'Reilly, Andrias O.; Burton, Mark J.; Puinean, Alin M.; Mellor, Ian R.; Duce, Ian R.; Field, Linda M.; Wallace, B.A.; Williamson, Martin S.; Davies, T.G. Emyr

Abstract

We describe the identification in aphids of a unique heterodimeric voltage-gated sodium channel which has an atypical ion selectivity filter and, unusually for insect channels, is highly insensitive to tetrodotoxin. We demonstrate that this channel has most likely arisen by adaptation (gene fission or duplication) of an invertebrate ancestral mono(hetero)meric channel. This is the only identifiable voltage-gated sodium channel homologue in the aphid genome(s), and the channel's novel selectivity filter motif (DENS instead of the usual DEKA found in other eukaryotes) may result in a loss of sodium selectivity, as indicated experimentally in mutagenised Drosophila channels.

Citation

Amey, J. S., O'Reilly, A. O., Burton, M. J., Puinean, A. M., Mellor, I. R., Duce, I. R., …Davies, T. E. (2015). An evolutionarily-unique heterodimeric voltage-gated cation channel found in aphids. FEBS Letters, 589(5), 598-607. https://doi.org/10.1016/j.febslet.2015.01.020

• Journal Article Type: Article
• Acceptance Date: Jan 5, 2015
• Online Publication Date: Jan 27, 2015
• Publication Date: Feb 27, 2015
• Deposit Date: Jul 15, 2016
• Publicly Available Date: Jul 15, 2016
• Journal: FEBS Letters
• Print ISSN: 0014-5793
• Electronic ISSN: 1873-3468
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 589
• Issue: 5
• Pages: 598-607
• DOI: https://doi.org/10.1016/j.febslet.2015.01.020
• Keywords: Voltage-gated sodium channel, Aphid, Evolution, Tetrodotoxin
• Public URL: https://nottingham-repository.worktribe.com/output/744243
• Publisher URL: http://onlinelibrary.wiley.com/doi/10.1016/j.febslet.2015.01.020/abstract
• Additional Information: This article is maintained by: Elsevier; Article Title: An evolutionarily-unique heterodimeric voltage-gated cation channel found in aphids; Journal Title: FEBS Letters; CrossRef DOI link to publisher maintained version: http://dx.doi.org/10.1016/j.febslet.2015.01.020; Content Type: article; Copyright: Copyright © 2015 The Authors. Published by Elsevier B.V.