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Factors affecting phage D29 infection: a tool to investigate different growth states of mycobacteria

Swift, Benjamin; Gerrard, Zara; Huxley, Jonathan; Rees, Catherine

Authors

Benjamin Swift benjamin.swift@nottingham.ac.uk

Zara Gerrard sbxzeg@nottingham.ac.uk

Jonathan Huxley jon.huxley@nottingham.ac.uk

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CATH REES cath.rees@nottingham.ac.uk
Associate Professor



Abstract

Bacteriophages D29 and TM4 are able to infect a wide range of mycobacteria, including pathogenic and non pathogenic species. Successful phage infection of both fast- and slow-growing mycobacteria can be rapidly detected using the phage amplification assay. Using this method, the effect of oxygen limitation during culture of mycobacteria on the success of phage infection was studied. Both D29 and TM4 were able to infect cultures of M. smegmatis and Mycobacterium avium subspecies paratuberculosis (MAP) grown in liquid with aeration. However when cultures were grown under oxygen limiting conditions, only TM4 could productively infect the cells. Cell attachment assays showed that D29 could bind to the cells surface but did not complete the lytic cycle. The ability of D29 to productively infect the cells was rapidly recovered (within 1 day) when the cultures were returned to an aerobic environment and this recovery required de novo RNA synthesis. These results indicated that under oxygen limiting conditions the cells are entering a growth state which inhibits phage D29 replication, and this change in host cell biology which can be detected by using both phage D29 and TM4 in the phage amplification assay.

Journal Article Type Article
Publication Date Sep 3, 2014
Journal PLoS ONE
Electronic ISSN 1932-6203
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 9
Issue 9
Article Number e106690
APA6 Citation Swift, B., Gerrard, Z., Huxley, J., & Rees, C. (2014). Factors affecting phage D29 infection: a tool to investigate different growth states of mycobacteria. PLoS ONE, 9(9), doi:10.1371/journal.pone.0106690
DOI https://doi.org/10.1371/journal.pone.0106690
Keywords bacteriophages, mycobacteria, phage amplification assay
Publisher URL http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0106690
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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