Campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation

Karlyshev, A.V.; Thacker, G.; Jones, M.A.; Clements, M.O.; Wren, Brendan W.

Abstract

According to MEROPS peptidase database, Campylobacter species encode 64 predicted peptidases. However, proteolytic properties of only a few of these proteins have been confirmed experimentally. In this study we identified and characterised a Campylobacter jejuni gene cj0511 encoding a novel peptidase. The proteolytic activity associated with this enzyme was demonstrated in cell lysates. Moreover, enzymatic studies conducted with a purified protein confirmed a prediction of it being a serine peptidase. Furthermore, cj0511 mutant was found to be severely attenuated in chicken colonisation model, suggesting a role of the Cj0511 protein in infection.

Citation

Karlyshev, A., Thacker, G., Jones, M., Clements, M., & Wren, B. W. (2014). Campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation. FEBS Open Bio, 4(1), https://doi.org/10.1016/j.fob.2014.04.012

• Journal Article Type: Article
• Acceptance Date: Apr 28, 2014
• Publication Date: May 9, 2014
• Deposit Date: Apr 6, 2017
• Publicly Available Date: Apr 6, 2017
• Journal: FEBS Open Bio
• Electronic ISSN: 2211-5463
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 4
• Issue: 1
• DOI: https://doi.org/10.1016/j.fob.2014.04.012
• Keywords: Campylobacter, Protease, Colonisation, Chicken
• Public URL: https://nottingham-repository.worktribe.com/output/728878
• Publisher URL: http://onlinelibrary.wiley.com/doi/10.1016/j.fob.2014.04.012/epdf
• Contract Date: Apr 6, 2017