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Campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation

Karlyshev, A.V.; Thacker, G.; Jones, M.A.; Clements, M.O.; Wren, Brendan W.

Authors

A.V. Karlyshev

G. Thacker

M.O. Clements

Brendan W. Wren brendan.wren@lshtm.ac.uk



Abstract

According to MEROPS peptidase database, Campylobacter species encode 64 predicted peptidases. However, proteolytic properties of only a few of these proteins have been confirmed experimentally. In this study we identified and characterised a Campylobacter jejuni gene cj0511 encoding a novel peptidase. The proteolytic activity associated with this enzyme was demonstrated in cell lysates. Moreover, enzymatic studies conducted with a purified protein confirmed a prediction of it being a serine peptidase. Furthermore, cj0511 mutant was found to be severely attenuated in chicken colonisation model, suggesting a role of the Cj0511 protein in infection.

Citation

Karlyshev, A., Thacker, G., Jones, M., Clements, M., & Wren, B. W. (2014). Campylobacter jejuni gene cj0511 encodes a serine peptidase essential for colonisation. FEBS Open Bio, 4(1), https://doi.org/10.1016/j.fob.2014.04.012

Journal Article Type Article
Acceptance Date Apr 28, 2014
Publication Date May 9, 2014
Deposit Date Apr 6, 2017
Publicly Available Date Apr 6, 2017
Journal FEBS Open Bio
Electronic ISSN 2211-5463
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 4
Issue 1
DOI https://doi.org/10.1016/j.fob.2014.04.012
Keywords Campylobacter, Protease, Colonisation, Chicken
Public URL http://eprints.nottingham.ac.uk/id/eprint/41765
Publisher URL http://onlinelibrary.wiley.com/doi/10.1016/j.fob.2014.04.012/epdf
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0





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