Is the atherosclerotic phenotype of pre-eclamptic placentas due to altered lipoprotein concentrations and placental lipoprotein receptors?: role of a small-for-gestational-age phenotype

Hentchke, Marta; Poli-de-Figueiredo, Carlos; Pinheiro da Costa, Bartira; Kurlak, L.O.; Williams, Paula Juliet; Mistry, Hiten D.

Abstract

Artherosis of spiral arteries in uteroplacental beds from pre-eclamptic women resemble those of atherosclerosis, characterised by increased plasma lipids and lipoproteins. We hypothesised 1) lipoproteins receptors/transporter in placenta would be up-regulated in pre-eclampsia, associated with increased maternal and fetal lipoprotein concentrations; 2) expression of these would be reduced in pre-eclamptic placentae from women delivering small-for-gestational-age (SGA) infants. Placental biopsies, maternal and umbilical serum samples were taken from 27 normotensive and 24 pre-eclamptic women. Maternal/umbilical cord serum LDL; HDL; total cholesterol and triglycerides were measured. Placental mRNA expression of lipoprotein receptors/transporters were quantified using qRT-PCR. Protein localisation/expression of LRP-1 in the pre-eclamptic with/without SGA was measured by immunohistochemistry. Placental mRNA expression of all genes except PON-1, MTTP and PDIA2 were observed. No differences for any lipoprotein receptors/transporters were found between groups; however, in the pre-eclamptic group placental LRP-1 expression was lower in SGA delivering mothers (n = 7; P=0.036). LRP-1 protein was localised around fetal vessels and Hofbauer cells. This is the first detailed study of maternal/fetal lipoprotein concentrations and placental lipoprotein receptor mRNA expression in normotensive and pre-eclamptic pregnancies. These findings do not support a role of altered lipid metabolism in pre-eclampsia, but may be involved in fetal growth.

Citation

Hentchke, M., Poli-de-Figueiredo, C., Pinheiro da Costa, B., Kurlak, L., Williams, P. J., & Mistry, H. D. (2013). Is the atherosclerotic phenotype of pre-eclamptic placentas due to altered lipoprotein concentrations and placental lipoprotein receptors?: role of a small-for-gestational-age phenotype. Journal of Lipid Research, 54, https://doi.org/10.1194/jlr.M036699

• Journal Article Type: Article
• Acceptance Date: Jul 16, 2013
• Online Publication Date: Jul 29, 2013
• Publication Date: Oct 31, 2013
• Deposit Date: Jul 21, 2017
• Publicly Available Date: Jul 21, 2017
• Journal: Journal of Lipid Research
• Print ISSN: 0022-2275
• Electronic ISSN: 1539-7262
• Publisher: American Society for Biochemistry and Molecular Biology
• Peer Reviewed: Peer Reviewed
• Volume: 54
• DOI: https://doi.org/10.1194/jlr.M036699
• Keywords: Hypertension; Lipids; LDL cholesterol; HDL cholesterol; Triglycerides; ABCA1; LRP-1; LDL-R; SRB-1;Fetal growth
• Public URL: https://nottingham-repository.worktribe.com/output/718252
• Publisher URL: http://www.jlr.org/content/54/10/2658
• Additional Information: This research was originally published in Journal of Lipid Research. Marta R. Hentschke, Carlos E. Poli-de-Figueiredo, Bartira E. Pinheiro da Costa, Lesia O. Kurlak, Paula J. Williams, Hiten D. Mistry . Is the atherosclerotic phenotype of pre-eclamptic placentas due to altered lipoprotein concentrations and placental lipoprotein receptors?: role of a small-for-gestational-age phenotype. Journal of Lipid Research. 2013; Vol: 54 p. 2658-2664. © the American Society for Biochemistry and Molecular Biology.
• Contract Date: Jul 21, 2017