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Exploration of temperature and shelf-life dependency of the therapeutically available Insulin Detemir

Beji, Oritsegidenene; Gillis, Richard B.; Dinu, Vlad; Jiwani, Shahwar I.; Gyasi-Antwi, Philemon; Fisk, Ian D.; Meal, Andrew; Morgan, Paul S.; Harding, Stephen E.; Huang, Sha; Agugini, Giulia; Fedele, Federica; Adams, Gary G.

Authors

Oritsegidenene Beji

Vlad Dinu

ANDY MEAL andy.meal@nottingham.ac.uk
Assistant Professor

Paul S. Morgan

Sha Huang

Giulia Agugini

Federica Fedele



Contributors

Charlotte Moody
Other

Abstract

© 2020 Elsevier B.V. Purpose: Insulin, in typical use, undergoes multiple changes in temperature; from refrigerator, to room temperature, to body temperature. Although long-term storage temperature has been well-studied, the short term changes to insulin are yet to be determined. Insulin detemir (IDet) is a clinically available, slow-acting, synthetic analogue characterised by the conjugation of a C14 fatty acid. The function of this modification is to cause the insulin to form multi-hexameric species, thus retarding the pharmacokinetic rate of action. In this investigation, the temperature dependence properties of this synthetic analogue is probed, as well as expiration. Methods: Dynamic light scattering (DLS) and viscometry were employed to assess the effect of temperature upon IDet. Mass spectrometry was also used to probe the impact of shelf-life and the presence of certain excipients. Results: IDet was compared with eight other insulins, including human recombinant, three fast-acting analogues and two other slow-acting analogues. Of all nine insulins, IDet was the only analogue to show temperature dependent behaviour, between 20 °C and 37 °C, when probed with non-invasive backscatter dynamic light scattering. Upon further investigation, IDet observed significant changes in size related to temperature, direction of temperature (heated/cooled) and expiration with cross-correlation observed amongst all 4 parameters. Conclusions: These findings are critical to our understanding of the behaviour of this particular clinically relevant drug, as it will allow the development of future generations of peptide-based therapies with greater clinical efficacy.

Citation

Beji, O., Gillis, R. B., Dinu, V., Jiwani, S. I., Gyasi-Antwi, P., Fisk, I. D., …Adams, G. G. (2020). Exploration of temperature and shelf-life dependency of the therapeutically available Insulin Detemir. European Journal of Pharmaceutics and Biopharmaceutics, 152, 340-347. https://doi.org/10.1016/j.ejpb.2020.05.016

Journal Article Type Article
Acceptance Date May 17, 2020
Online Publication Date May 21, 2020
Publication Date Jul 1, 2020
Deposit Date May 29, 2020
Publicly Available Date May 22, 2021
Journal European Journal of Pharmaceutics and Biopharmaceutics
Print ISSN 0939-6411
Electronic ISSN 1873-3441
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 152
Pages 340-347
DOI https://doi.org/10.1016/j.ejpb.2020.05.016
Keywords Biotechnology; Pharmaceutical Science; General Medicine
Public URL https://nottingham-repository.worktribe.com/output/4529760
Publisher URL https://www.sciencedirect.com/science/article/pii/S0939641120301429