Peritoneal expression of Matrilysin helps identify early post-operative recurrence of colorectal cancer

Gaspari, Achille L.; Sica, Giuseppe S.; Iaculli, Edoardo; Fiorani, Cristina; Tema, Giorgia; Stolfi, Carmine; Divizia, Andrea; Monteleone, Giovanni; Sibio, Simone; Candi, Eleonora; Catani, Valeria; Amelio, Ivano

Abstract

Recurrence of colorectal cancer (CRC) following a potentially curative resection is a challenging clinical problem. Matrix metalloproteinase-7 (MMP-7) is over-expressed by CRC cells and supposed to play a major role in CRC cell diffusion and metastasis. MMP-7 RNA expression was assessed by real-time PCR using specific primers in peritoneal washing fluid obtained during surgical procedure. After surgery, patients underwent a regular follow up for assessing recurrence. transcripts for MMP-7 were detected in 31/57 samples (54%). Patients were followed-up (range 20–48 months) for recurrence prevention. Recurrence was diagnosed in 6 out of 55 patients (11%) and two patients eventually died because of this. Notably, all the six patients who had relapsed were positive for MMP-7. Sensitivity and specificity of the test were 100% and 49% respectively. Data from patients have also been corroborated by computational approaches. Public available coloncarcinoma datasets have been employed to confirm MMP7 clinical impact on the disease. Interestingly, MMP-7 expression appeared correlated to Tgfb-1, and correlation of the two factors represented a poor prognostic factor. This study proposes positivity of MMP-7 in peritoneal cavity as a novel biomarker for predicting disease recurrence in patients with CRC.

• Journal Article Type: Article
• Acceptance Date: Nov 27, 2014
• Online Publication Date: Jan 9, 2015
• Publication Date: May 30, 2015
• Deposit Date: Apr 1, 2020
• Publicly Available Date: May 4, 2020
• Journal: Oncotarget
• Publisher: Impact Journals
• Peer Reviewed: Peer Reviewed
• Volume: 6
• Issue: 15
• Pages: 13402-13415
• DOI: https://doi.org/10.18632/oncotarget.2830
• Public URL: https://nottingham-repository.worktribe.com/output/4236654
• Publisher URL: https://www.oncotarget.com/article/2830/text/