Expression of different types of [FeFe]-hydrogenase genes in bacteria isolated from a population of a bio-hydrogen pilot-scale plant

Morra, Simone; Arizzi, Mariaconcetta; Allegra, Paola; La Licata, Barbara; Sagnelli, Fabio; Zitella, Paola; Gilardi, Gianfranco; Valetti, Francesca

Abstract

[FeFe]-hydrogenases are the enzymes responsible for high yield H 2 production during dark fermentation in bio-hydrogen production plants. The culturable bacterial population present in a pilot-scale plant efficiently producing H2 from waste materials was isolated, classified and identified by means of 16S rDNA gene analysis. The culturable part of the mixed population consists of nine bacterial species that include non-hydrogen producers (Lactobacillus, Enterococcus and Staphylococcus) and several Clostridium that are directly responsible for H2 production. An extensive analysis of the expression of [FeFe]-hydrogenases in the three best producer strains was achieved by RT-PCR, covering the complete set of known genes for each species. This revealed that during H2 production there are several different [FeFe]-hydrogenases simultaneously expressed, with genes belonging to the same phylogenetic and structural classification sharing similar transcriptional profiles. © 2014, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.

Citation

Morra, S., Arizzi, M., Allegra, P., La Licata, B., Sagnelli, F., Zitella, P., Gilardi, G., & Valetti, F. (2014). Expression of different types of [FeFe]-hydrogenase genes in bacteria isolated from a population of a bio-hydrogen pilot-scale plant. International Journal of Hydrogen Energy, 39(17), 9018-9027. https://doi.org/10.1016/j.ijhydene.2014.04.009

• Journal Article Type: Article
• Acceptance Date: Apr 1, 2014
• Online Publication Date: Apr 26, 2014
• Publication Date: Jun 5, 2014
• Deposit Date: Jan 31, 2020
• Publicly Available Date: Feb 3, 2020
• Journal: International Journal of Hydrogen Energy
• Print ISSN: 0360-3199
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 39
• Issue: 17
• Pages: 9018-9027
• DOI: https://doi.org/10.1016/j.ijhydene.2014.04.009
• Keywords: Fuel Technology; Renewable Energy, Sustainability and the Environment; Energy Engineering and Power Technology; Condensed Matter Physics
• Public URL: https://nottingham-repository.worktribe.com/output/3841476
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0360319914010003
• Additional Information: This article is maintained by: Elsevier; Article Title: Expression of different types of [FeFe]-hydrogenase genes in bacteria isolated from a population of a bio-hydrogen pilot-scale plant; Journal Title: International Journal of Hydrogen Energy; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.ijhydene.2014.04.009; Content Type: article; Copyright: Copyright © 2014 Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.