Oxygen-resistant [FeFe]hydrogenases: new biocatalysis tools for clean energy and cascade reactions

Valetti, Francesca; Morra, Simone; Barbieri, Lisa; Dezzani, Sabrina; Ratto, Alessandro; Catucci, Gianluca; Sadeghi, Sheila J.; Gilardi, Gianfranco

Abstract

The use of enzymes to generate hydrogen, instead of using rare metal catalysts, is an exciting area of study in modern biochemistry and biotechnology, as well as biocatalysis driven by sustainable hydrogen. Thus far, the oxygen sensitivity of the fastest hydrogen-producing/exploiting enzymes, [FeFe]hydrogenases, has hindered their practical application, thereby restricting innovations mainly to their [NiFe]-based, albeit slower, counterparts. Recent exploration of the biodiversity of clostridial hydrogen-producing enzymes has yielded the isolation of representatives from a relatively understudied group. These enzymes possess an inherent defense mechanism against oxygen-induced damage. This discovery unveils fresh opportunities for applications such as electrode interfacing, biofuel cells, immobilization, and entrapment for enhanced stability in practical uses. Furthermore, it suggests potential combinations with cascade reactions for CO2 conversion or cofactor regeneration, like NADPH, facilitating product separation in biotechnological processes. This work provides an overview of this new class of biocatalysts, incorporating unpublished protein engineering strategies to further investigate the dynamic mechanism of oxygen protection and to address crucial details remaining elusive such as still unidentified switching hot-spots and their effects. Variants with improved kcat as well as chimeric versions with promising features to attain gain-of-function variants and applications in various biotechnological processes are also presented.

• Journal Article Type: Article
• Acceptance Date: Feb 21, 2024
• Online Publication Date: Feb 21, 2024
• Publication Date: 2024
• Deposit Date: Apr 19, 2024
• Publicly Available Date: Jun 14, 2024
• Journal: Faraday Discussions
• Print ISSN: 1359-6640
• Electronic ISSN: 1364-5498
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• DOI: https://doi.org/10.1039/d4fd00010b
• Keywords: Physical and Theoretical Chemistry
• Public URL: https://nottingham-repository.worktribe.com/output/33838034
• Publisher URL: https://pubs.rsc.org/en/content/articlelanding/2024/fd/d4fd00010b