Expression and role of CYP505A1 in pathogenicity of Fusarium oxysporum f. sp. lactucae

Minerdi, Daniela; Sadeghi, Sheila J.; Pautasso, Lara; Morra, Simone; Aigotti, Riccardo; Medana, Claudio; Gilardi, Giovanna; Gullino, Maria Lodovica; Gilardi, Gianfranco

Abstract

Background: Cytochrome P450 enzymes (CYPs) are monooxygenases present in every domain of life. In fungi
CYPs are involved in virulence. Fusarium wilt of lettuce, caused by F. oxysporum f. sp. lactucae, is the most
serious disease of lettuce. F. oxysporum f.sp. lactucae MSA35 is an antagonistic fungus. Pathogenic formae specialis
of F. oxysporum possess a CYP belonging to the new family CYP505. This enzyme hydroxylates saturated fatty
acids that play a role in plant defence.
Methods: Molecular tools were adopted to search for cyp505 gene in MSA35 genome. cyp505 gene expression
analysis in pathogenic and antagonistic Fusarium was performed. The enzyme was expressed in its recombinant
form and used for catalytic reactions with fatty acids, the products of which were characterized by mass spectrometry
analysis.
Results: A novel MSA35 self-sufficient CYP505 is differentially expressed in antagonistic and pathogenic F.
oxysporum. Its expression is induced by the host plant lettuce in both pathogenesis and antagonism during the
early phase of the interaction, while it is silenced during the late phase only in antagonistic Fusarium. Massspectrometry
investigations proved that CYP505A1 mono-hydroxylates lauric, palmitic and stearic acids.
Conclusions: The ability of CYP505A1 to oxidize fatty acids present in the cortical cell membranes together with
its differential expression in its Fusarium antagonistic form point out to the possibility that this enzyme is associated
with Fusarium pathogenicity in lettuce.
General significance: The CYP505 clan is present in pathogenic fungal phyla, making CYP505A1 enzyme a putative
candidate as a new target for the development of novel antifungal molecules.

Citation

Minerdi, D., Sadeghi, S. J., Pautasso, L., Morra, S., Aigotti, R., Medana, C., …Gilardi, G. (2020). Expression and role of CYP505A1 in pathogenicity of Fusarium oxysporum f. sp. lactucae. BBA - Proteins and Proteomics, 1868(1), Article 140268. https://doi.org/10.1016/j.bbapap.2019.140268

• Journal Article Type: Article
• Acceptance Date: Sep 1, 2019
• Online Publication Date: Sep 3, 2019
• Publication Date: 2020-01
• Deposit Date: Jan 31, 2020
• Journal: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
• Print ISSN: 1570-9639
• Electronic ISSN: 1878-1454
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 1868
• Issue: 1
• Article Number: 140268
• DOI: https://doi.org/10.1016/j.bbapap.2019.140268
• Keywords: Biophysics; Analytical Chemistry; Biochemistry; Molecular Biology
• Public URL: https://nottingham-repository.worktribe.com/output/3840991
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S1570963919301542
• Additional Information: This article is maintained by: Elsevier; Article Title: Expression and role of CYP505A1 in pathogenicity of Fusarium oxysporum f. sp. lactucae; Journal Title: Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.bbapap.2019.140268; Content Type: article; Copyright: © 2019 Elsevier B.V. All rights reserved.