William J. Dalleywater
Tissue transglutaminase treatment leads to concentration-dependent changes in dendritic cell phenotype - implications for the role of transglutaminase in coeliac disease
Dalleywater, William J.; Chau, David Y.S.; Ghaemmaghami, Amir M.
Authors
David Y.S. Chau
Professor AMIR GHAEMMAGHAMI AMIR.GHAEMMAGHAMI@NOTTINGHAM.AC.UK
Professor of Immunology and Immuno- Bioengineering
Abstract
Dendritic cells (DCs) are part of the innate immune system with a key role in initiating and modulating T cell mediated immune responses. Coeliac disease is caused by inappropriate activation of such a response leading to small intestinal inflammation when gluten is ingested. Tissue transglutaminase, an extracellular matrix (ECM) protein, has an established role in coeliac disease; however, little work to date has examined its impact on DCs. The aim of this study was to investigate the effect of small intestinal ECM proteins, fibronectin (FN) and tissue transglutaminase 2 (TG-2), on human DCs by including these proteins in DC cultures.The study used flow cytometry and scanning electron microscopy to determine the effect of FN and TG-2 on phenotype, endocytic ability and and morphology of DCs. Furthermore, DCs treated with FN and TG-2 were cultured with T cells and subsequent T cell proliferation and cytokine profile was determined.The data indicate that transglutaminase affected DCs in a concentration-dependent manner. High concentrations were associated with a more mature phenotype and increased ability to stimulate T cells, while lower concentrations led to maintenance of an immature phenotype.These data provide support for an additional role for transglutaminase in coeliac disease and demonstrate the potential of in vitro modelling of coeliac disease pathogenesis. © 2012 Dalleywater et al; licensee BioMed Central Ltd.
Citation
Dalleywater, W. J., Chau, D. Y., & Ghaemmaghami, A. M. (2012). Tissue transglutaminase treatment leads to concentration-dependent changes in dendritic cell phenotype - implications for the role of transglutaminase in coeliac disease. BMC Immunology, 13, Article 20. https://doi.org/10.1186/1471-2172-13-20
Journal Article Type | Article |
---|---|
Acceptance Date | Apr 16, 2012 |
Online Publication Date | Apr 16, 2012 |
Publication Date | Apr 16, 2012 |
Deposit Date | Jan 3, 2023 |
Publicly Available Date | Jan 23, 2023 |
Journal | BMC Immunology |
Electronic ISSN | 1471-2172 |
Publisher | Springer Verlag |
Peer Reviewed | Peer Reviewed |
Volume | 13 |
Article Number | 20 |
DOI | https://doi.org/10.1186/1471-2172-13-20 |
Public URL | https://nottingham-repository.worktribe.com/output/3097491 |
Publisher URL | https://bmcimmunol.biomedcentral.com/articles/10.1186/1471-2172-13-20 |
Files
1471-2172-13-20
(1.2 Mb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by/2.0/
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