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A randomized, controlled, double-blind crossover study on the effects of isoeffective and isovolumetric intravenous crystalloid and gelatin on blood volume, and renal and cardiac hemodynamics

Bradley, Christopher R.; Bragg, Damian D.; Cox, Eleanor F.; El-Sharkawy, Ahmed M.; Buchanan, Charlotte E.; Chowdhury, Abeed H.; Macdonald, Ian A.; Francis, Susan T.; Lobo, Dileep N.


Christopher R. Bradley

Damian D. Bragg

Senior Research Fellow

Ahmed M. El-Sharkawy

Abeed H. Chowdhury

Ian A. Macdonald

Professor of Gastrointestinal Surgery


Background & aims
Blood volume expanding properties of colloids are superior to crystalloids. In addition to oncotic/osmotic properties, the electrolyte composition of infusions may have important effects on visceral perfusion, with infusions containing supraphysiological chloride causing hyperchloremic acidosis and decreased renal blood flow. In this non-inferiority study, a validated healthy human subject model was used to compare effects of colloid (4% succinylated gelatin) and crystalloid fluid regimens on blood volume, renal function, and cardiac output.

Healthy male participants were given infusions over 60 min > 7 days apart in a randomized, crossover manner. Reference arm (A): 1.5 L of Sterofundin ISO, isoeffective arm (B): 0.5 L of 4% Gelaspan®, isovolumetric arm (C): 0.5 L of 4% Gelaspan® and 1 L of Sterofundin ISO (all B. Braun, Melsungen, Germany). Participants were studied over 240 min. Changes in blood volume were calculated from changes in weight and hematocrit. Renal volume, renal artery blood flow (RABF), renal cortex perfusion and diffusion, and cardiac index were measured with magnetic resonance imaging.

Ten of 12 males [mean (SE) age 23.9 (0.8) years] recruited, completed the study. Increase in body weight and extracellular fluid volume were significantly less after infusion B than infusions A and C, but changes in blood volume did not significantly differ between infusions. All infusions increased renal volume, with no significant differences between infusions. There was no significant difference in RABF across the infusion time course or between infusion types. Renal cortex perfusion decreased during the infusion (mean 18% decrease from baseline), with no significant difference between infusions. There was a trend for increased renal cortex diffusion (4.2% increase from baseline) for the crystalloid infusion. All infusions led to significant increases in cardiac index.

A smaller volume of colloid (4% succinylated gelatin) was as effective as a larger volume of crystalloid at expanding blood volume, increasing cardiac output and changing renal function. Significantly less interstitial space expansion occurred with the colloid.


Bradley, C. R., Bragg, D. D., Cox, E. F., El-Sharkawy, A. M., Buchanan, C. E., Chowdhury, A. H., …Lobo, D. N. (2020). A randomized, controlled, double-blind crossover study on the effects of isoeffective and isovolumetric intravenous crystalloid and gelatin on blood volume, and renal and cardiac hemodynamics. Clinical Nutrition, 39(7), 2070-2079.

Journal Article Type Article
Acceptance Date Sep 30, 2019
Online Publication Date Oct 16, 2019
Publication Date 2020-07
Deposit Date Oct 11, 2019
Journal Clinical Nutrition
Print ISSN 0261-5614
Electronic ISSN 1532-1983
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 39
Issue 7
Pages 2070-2079
Keywords Balanced crystalloids; Colloids; Balanced gelatin solution; Gelaspan®; Sterofundin® ISO; healthy participants; Magnetic resonance imaging; Randomized controlled study; Cardiac output; Renal blood flow
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