Jordan Whisler
Emergent mechanical control of vascular morphogenesis
Whisler, Jordan; Shahreza, Somayeh; Schlegelmilch, Karin; Ege, Nil; Javanmardi, Yousef; Malandrino, Andrea; Agrawal, Ayushi; Fantin, Alessandro; Serwinski, Bianca; Azizgolshani, Hesham; Park, Clara; Shone, Victoria; Demuren, Olukunle O.; Del Rosario, Amanda; Butty, Vincent L.; Holroyd, Natalie; Domart, Marie-Charlotte; Hooper, Steven; Szita, Nicolas; Boyer, Laurie A.; Walker-Samuel, Simon; Djordjevic, Boris; Sheridan, Graham K.; Collinson, Lucy; Calvo, Fernando; Ruhrberg, Christiana; Sahai, Erik; Kamm, Roger; Moeendarbary, Emad
Authors
Somayeh Shahreza
Karin Schlegelmilch
Nil Ege
Yousef Javanmardi
Andrea Malandrino
Ayushi Agrawal
Alessandro Fantin
Bianca Serwinski
Hesham Azizgolshani
Clara Park
Victoria Shone
Olukunle O. Demuren
Amanda Del Rosario
Vincent L. Butty
Natalie Holroyd
Marie-Charlotte Domart
Steven Hooper
Nicolas Szita
Laurie A. Boyer
Simon Walker-Samuel
Boris Djordjevic
Dr GRAHAM SHERIDAN GRAHAM.SHERIDAN@NOTTINGHAM.AC.UK
Assistant Professor
Lucy Collinson
Fernando Calvo
Christiana Ruhrberg
Erik Sahai
Roger Kamm
Emad Moeendarbary
Abstract
Vascularization is driven by morphogen signals and mechanical cues that coordinately regulate cellular force generation, migration, and shape change to sculpt the developing vascular network. However, it remains unclear whether developing vasculature actively regulates its own mechanical properties to achieve effective vascularization. We engineered tissue constructs containing endothelial cells and fibroblasts to investigate the mechanics of vascularization. Tissue stiffness increases during vascular morphogenesis resulting from emergent interactions between endothelial cells, fibroblasts, and ECM and correlates with enhanced vascular function. Contractile cellular forces are key to emergent tissue stiffening and synergize with ECM mechanical properties to modulate the mechanics of vascularization. Emergent tissue stiffening and vascular function rely on mechanotransduction signaling within fibroblasts, mediated by YAP1. Mouse embryos lacking YAP1 in fibroblasts exhibit both reduced tissue stiffness and develop lethal vascular defects. Translating our findings through biology-inspired vascular tissue engineering approaches will have substantial implications in regenerative medicine.
Journal Article Type | Article |
---|---|
Acceptance Date | Jul 13, 2023 |
Publication Date | Aug 11, 2023 |
Deposit Date | Jan 31, 2024 |
Publicly Available Date | Jan 31, 2024 |
Journal | Science Advances |
Publisher | American Association for the Advancement of Science |
Peer Reviewed | Peer Reviewed |
Volume | 9 |
Issue | 32 |
Article Number | eadg9781 |
DOI | https://doi.org/10.1126/sciadv.adg9781 |
Keywords | Multidisciplinary |
Public URL | https://nottingham-repository.worktribe.com/output/24424136 |
Publisher URL | https://www.science.org/doi/10.1126/sciadv.adg9781 |
Files
sciadv.adg9781
(2 Mb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
You might also like
Poroelastic osmoregulation of living cell volume
(2021)
Journal Article
Tumour brain: pre‐treatment cognitive and affective disorders caused by peripheral cancers
(2021)
Journal Article
Extracellular Vesicles and Intercellular Communication in the Central Nervous System
(2021)
Journal Article
Mechanobiology of the brain in ageing and Alzheimer's disease
(2020)
Journal Article
Downloadable Citations
About Repository@Nottingham
Administrator e-mail: discovery-access-systems@nottingham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search