Parallel imaging with phonon microscopy using a multi-core fibre bundle detection

Fuentes-Domínguez, Rafael; Yao, Mengting; Hardiman, William; La Cavera III, Salvatore; Setchfield, Kerry; Pérez-Cota, Fernando; Smith, Richard J.; Clark, Matt

Abstract

In this paper, we show a proof-of-concept method to parallelise phonon microscopy measurements for cell elasticity imaging by demonstrating a 3-fold increase in acquisition speed which is limited by current acquisition hardware. Phonon microscopy is based on time-resolved Brillouin scattering, which uses a pump–probe method with asynchronous optical sampling (ASOPS) to generate and detect coherent phonons. This enables access to the cell elasticity via the Brillouin frequency with sub-optical axial resolution. Although systems based on ASOPS are typically faster compared to the ones built with a mechanical delay line, they are still very slow to study real time changes at the cellular level. Additionally, the biocompatibility is reduced due to long light exposure and scanning time. Using a multi-core fibre bundle rather than a single channel for detection, we acquire 6 channels simultaneously allowing us to speed-up measurements, and open a way to scale-up this method.

• Journal Article Type: Article
• Acceptance Date: Apr 11, 2023
• Online Publication Date: Apr 24, 2023
• Publication Date: 2023-06
• Deposit Date: May 9, 2023
• Publicly Available Date: May 11, 2023
• Journal: Photoacoustics
• Publisher: Elsevier BV
• Peer Reviewed: Peer Reviewed
• Volume: 31
• Article Number: 100493
• DOI: https://doi.org/10.1016/j.pacs.2023.100493
• Keywords: Picosecond ultrasonics; Phonon microscopy; Time-domain Brillouin scattering; Parallel measurements
• Public URL: https://nottingham-repository.worktribe.com/output/20008704
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S2213597923000460?via%3Dihub