Low Molecular Weight Nucleoside Gelators: A Platform for Protein Aggregation Inhibition

Johnson, Litty; Faidra Angelerou, Maria Galini; Surikutchi, Bhanu Teja; Allen, Stephanie; Zelzer, Mischa; Marlow, Maria

doi:10.1021/acs.molpharmaceut.8b01013

Authors

Litty Johnson

Maria Galini Faidra Angelerou

Bhanu Teja Surikutchi

STEPHANIE ALLEN stephanie.allen@nottingham.ac.uk
Professor of Pharmaceutical Biophysics

MISCHA ZELZER M.Zelzer@nottingham.ac.uk
Associate Professor

MARIA MARLOW Maria.Marlow@nottingham.ac.uk
Associate Professor

Abstract

© Copyright 2018 American Chemical Society. Low molecular weight nucleoside gelators hold great promise in drug delivery and particularly for the delivery of biologics because of their excellent biocompatibility. However, the influence of these gelators on protein aggregation inhibition has not yet been studied. Protein aggregation is the most significant cause of protein instability and can severely impact the biological activity of the protein, impairing the quality and safety of the formulation. Herein, we report the ability of a nucleoside-based gelator, N4-octanoyl-2′-deoxycytidine, to inhibit protein aggregation. Using turbidimetric, spectroscopic, and microscopic methods, we demonstrate that protein aggregation inhibition is dependent on gelator concentration. Moreover, we have found that the protein is still functionally active in the hydrogel.

Citation

Johnson, L., Faidra Angelerou, M. G., Surikutchi, B. T., Allen, S., Zelzer, M., & Marlow, M. (2019). Low Molecular Weight Nucleoside Gelators: A Platform for Protein Aggregation Inhibition. Molecular Pharmaceutics, 16(1), 462-467. https://doi.org/10.1021/acs.molpharmaceut.8b01013

Journal Article Type	Article
Acceptance Date	Nov 29, 2018
Online Publication Date	Nov 29, 2018
Publication Date	Jan 7, 2019
Deposit Date	Dec 10, 2018
Publicly Available Date	Nov 30, 2019
Journal	Molecular Pharmaceutics
Print ISSN	1543-8384
Electronic ISSN	1543-8392
Publisher	American Chemical Society
Peer Reviewed	Peer Reviewed
Volume	16
Issue	1
Pages	462-467
DOI	https://doi.org/10.1021/acs.molpharmaceut.8b01013
Keywords	Molecular Medicine; Drug Discovery; Pharmaceutical Science
Public URL	https://nottingham-repository.worktribe.com/output/1401804
Publisher URL	https://pubs.acs.org/doi/10.1021/acs.molpharmaceut.8b01013