Low Molecular Weight Nucleoside Gelators: A Platform for Protein Aggregation Inhibition

Johnson, Litty; Faidra Angelerou, Maria Galini; Surikutchi, Bhanu Teja; Allen, Stephanie; Zelzer, Mischa; Marlow, Maria

Abstract

© Copyright 2018 American Chemical Society. Low molecular weight nucleoside gelators hold great promise in drug delivery and particularly for the delivery of biologics because of their excellent biocompatibility. However, the influence of these gelators on protein aggregation inhibition has not yet been studied. Protein aggregation is the most significant cause of protein instability and can severely impact the biological activity of the protein, impairing the quality and safety of the formulation. Herein, we report the ability of a nucleoside-based gelator, N4-octanoyl-2′-deoxycytidine, to inhibit protein aggregation. Using turbidimetric, spectroscopic, and microscopic methods, we demonstrate that protein aggregation inhibition is dependent on gelator concentration. Moreover, we have found that the protein is still functionally active in the hydrogel.

Citation

Johnson, L., Faidra Angelerou, M. G., Surikutchi, B. T., Allen, S., Zelzer, M., & Marlow, M. (2019). Low Molecular Weight Nucleoside Gelators: A Platform for Protein Aggregation Inhibition. Molecular Pharmaceutics, 16(1), 462-467. https://doi.org/10.1021/acs.molpharmaceut.8b01013

• Journal Article Type: Article
• Acceptance Date: Nov 29, 2018
• Online Publication Date: Nov 29, 2018
• Publication Date: Jan 7, 2019
• Deposit Date: Dec 10, 2018
• Publicly Available Date: Nov 30, 2019
• Journal: Molecular Pharmaceutics
• Print ISSN: 1543-8384
• Electronic ISSN: 1543-8392
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 16
• Issue: 1
• Pages: 462-467
• DOI: https://doi.org/10.1021/acs.molpharmaceut.8b01013
• Keywords: Molecular Medicine; Drug Discovery; Pharmaceutical Science
• Public URL: https://nottingham-repository.worktribe.com/output/1401804
• Publisher URL: https://pubs.acs.org/doi/10.1021/acs.molpharmaceut.8b01013
• Contract Date: Dec 11, 2018